Question 1

A (n)  __________ vector is a plasmid that can be replicated in several different organisms because it has at least one origin of replication that will function in each host.

Accepted Answer

A)   shuttle
B)   chimeric
C)   expression
D)   phageE) None of the above
F) B) and C)

Question 2

Which of the following best describes the basis for separation of DNA fragments during agarose gel electrophoresis?

Accepted Answer

A)   The fragments with the highest percentage of G and C will migrate fastest.
B)   The fragments with the highest percentage of A and T will migrate fastest.
C)   The largest fragments will migrate fastest.
D)   The smallest fragments will migrate fastest.E) None of the above
F) A) and B)

Question 3

Which of the following is not part of a yeast artificial chromosome (YAC) ?

Accepted Answer

A)   the F factor
B)   a selectable marker
C)   an ARS
D)   a CEN sequenceE) B) and C)
F) All of the above

Question 4

A __________ is a DNA molecule used in hybridization reactions to detect the presence of a particular gene in separated DNA fragments.

Accepted Answer

A)   plasmid
B)   vector
C)   probe
D)   blotE) A) and D)
F) C) and D)

Question 5

Some plasmid vectors have incorporated the regulatory sequences of the lactose operon so that the expression of the recombinant gene can be induced at the appropriate time.

Accepted Answer

A) True 
 B)False

Question 6

The most frequently chosen prokaryotic host in cloning techniques is

Accepted Answer

A)   Saccharomyces cerevisiae
B)   Bacillus subtilis
C)   Staphylococcus aureus
D)   Escherichia coliE) B) and D)
F) All of the above

Question 7

The Southern blotting technique for transferring DNA from an agarose gel to a piece of nitrocellulose membrane is named for
E. M. Southern, the person who developed the procedure.

Accepted Answer

A) True 
 B)False

Question 8

A PCR procedure that allows a determination of the amount of a particular DNA fragment that is present in a sample is called

Accepted Answer

A)   quantitative PCR.
B)   analytical PCR.
C)   real-time PCR.
D)   reverse PCR.E) A) and B)
F) A) and C)

Question 9

Plasmid cloning vector DNA is usually introduced into bacterial hosts by

Accepted Answer

A)   ligation.
B)   transformation.
C)   transduction.
D)   plasmolysis.E) None of the above
F) A) and C)

Question 10

Cosmids are plasmids that can be packaged into capsids of the bacteriophage lambda; therefore, they can be transmitted like phages, but they can exist and replicate in a cell like plasmids.

Accepted Answer

A) True 
 B)False

Question 11

The three steps that take place in each cycle during PCR occur in which order?

Accepted Answer

A)   DNA annealing, denaturation, and synthesis
B)   DNA denaturation, annealing, and synthesis
C)   DNA synthesis, denaturation, and annealing
D)   none of the aboveE) A) and B)
F) None of the above

Question 12

Which of the following types of cloning vector can carry the largest amount of foreign DNA?

Accepted Answer

A)   bacterial artificial chromosome
B)   bacteriophage
C)   cosmid
D)   plasmidE) C) and D)
F) All of the above

Question 13

Which of the following can be used as vectors for cloning DNA fragments?

Accepted Answer

A)   plasmids
B)   cosmids
C)   bacteriophages
D)   all of the choicesE) None of the above
F) A) and B)

Question 14

One of the major advantages to using plasmids as cloning vectors is that very high copy numbers can be achieved with many types of plasmid vectors.

Accepted Answer

A) True 
 B)False

Question 15

Genetic engineering methods have been used to produce vaccines.

Accepted Answer

A) True 
 B)False

Question 16

___________ is a bacterial plasmid vector.

Accepted Answer

A)   Lambda
B)   T4 DNA ligase
C)   pUC19
D)   SV40E) None of the above
F) A) and D)

Question 17

Cloning a gene involves all of the following except

Accepted Answer

A)   isolating the fragment of DNA containing the desired gene.
B)   insertion of the gene into an appropriate vector.
C)   expression of the vector and the gene in a cell-free environment.
D)   introducing ligated DNA into
E)   coli cells.F) A) and E)
G) A) and B)

Question 18

A genomic __________ is a sufficiently large collection of recombinant DNA molecules in which the inserted sequences together represent the entire genome of an organism.

Accepted Answer

The answer of A genomic __________ is a sufficiently large...

Question 19

A DNA molecule used to carry a foreign gene into a host organism is called a

Accepted Answer

A)   plasmid.
B)   vector.
C)   probe.
D)   blot.E) All of the above
F) A) and D)

Question 20

Promoters for genes that code for proteins can be isolated from a cDNA library.

Accepted Answer

A) True 
 B)False

Exam 15: Recombinant Dna Technology

The three steps that take place in each cycle during PCR occur in which order?

Correct AnswerverifiedShow Answer

A DNA molecule used to carry a foreign gene into a host organism is called a

Correct AnswerverifiedShow Answer

Cosmids are plasmids that can be packaged into capsids of the bacteriophage lambda; therefore, they can be transmitted like phages, but they can exist and replicate in a cell like plasmids.

Correct AnswerverifiedShow Answer

Some plasmid vectors have incorporated the regulatory sequences of the lactose operon so that the expression of the recombinant gene can be induced at the appropriate time.

Correct AnswerverifiedShow Answer

One of the major advantages to using plasmids as cloning vectors is that very high copy numbers can be achieved with many types of plasmid vectors.

Correct AnswerverifiedShow Answer

A __________ is a DNA molecule used in hybridization reactions to detect the presence of a particular gene in separated DNA fragments.

Correct AnswerverifiedShow Answer

Which of the following is not part of a yeast artificial chromosome (YAC) ?

Correct AnswerverifiedShow Answer

___________ is a bacterial plasmid vector.

Correct AnswerverifiedShow Answer

A (n) __________ vector is a plasmid that can be replicated in several different organisms because it has at least one origin of replication that will function in each host.

Correct AnswerverifiedShow Answer

Genetic engineering methods have been used to produce vaccines.

Correct AnswerverifiedShow Answer

The most frequently chosen prokaryotic host in cloning techniques is

Correct AnswerverifiedShow Answer

Which of the following can be used as vectors for cloning DNA fragments?

Correct AnswerverifiedShow Answer

The Southern blotting technique for transferring DNA from an agarose gel to a piece of nitrocellulose membrane is named for E. M. Southern, the person who developed the procedure.

Correct AnswerverifiedShow Answer

A PCR procedure that allows a determination of the amount of a particular DNA fragment that is present in a sample is called

Correct AnswerverifiedShow Answer

Plasmid cloning vector DNA is usually introduced into bacterial hosts by

Correct AnswerverifiedShow Answer

Cloning a gene involves all of the following except

Correct AnswerverifiedShow Answer

Which of the following types of cloning vector can carry the largest amount of foreign DNA?

Correct AnswerverifiedShow Answer

A genomic __________ is a sufficiently large collection of recombinant DNA molecules in which the inserted sequences together represent the entire genome of an organism.

Correct AnswerverifiedShow Answer

Which of the following best describes the basis for separation of DNA fragments during agarose gel electrophoresis?

Correct AnswerverifiedShow Answer

Promoters for genes that code for proteins can be isolated from a cDNA library.

Correct AnswerverifiedShow Answer

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