Question 1

An enzyme that cleaves internal phosphodiester bonds of a DNA molecule is a (n)

Accepted Answer

A)  exonuclease.
B)  endonuclease.
C)  ligase.
D)  methylase.E) A) and B)
F) B) and C)

Question 2

A genomic __________ is a sufficiently large collection of recombinant DNA molecules in which the inserted sequences together represent the entire genome of an organism.

Accepted Answer

The answer of A genomic __________ is a sufficiently large...

Question 3

Cosmids are so named because they can be used to express foreign genes in a variety of different hosts.

Accepted Answer

A) True 
 B)False

Question 4

Antibiotics incorporated into the culture medium can

Accepted Answer

A)  select against organisms that have not incorporated the plasmid.
B)  select against organisms that have incorporated a plasmid not containing the desired gene.
C)  enhance production of recombinant proteins.
D)  select against organisms that have not incorporated the plasmid and select against organisms that have incorporated a plasmid not containing the desired geneE) B) and C)
F) A) and D)

Question 5

Restriction endonucleases are produced by

Accepted Answer

A)  fungi.
B)  bacteria.
C)  protozoa.
D)  plants.
E)  all of the choicesF) A) and E)
G) A) and D)

Question 6

___________ is a bacterial plasmid vector.

Accepted Answer

A)  Lambda
B)  T4 DNA ligase
C)  pUC19
D)  SV40E) All of the above
F) A) and C)

Question 7

Movement of charged molecules in an electrical field,which is used to separate nucleic acid fragments for recombinant DNA work,is called

Accepted Answer

A)  iontophoresis.
B)  nucleophoresis.
C)  electrophoresis.
D)  plasmaphoresis.E) A) and B)
F) B) and D)

Question 8

A __________ is a DNA molecule used in hybridization reactions to detect the presence of a particular gene in separated DNA fragments.

Accepted Answer

A)  plasmid
B)  vector
C)  probe
D)  blotE) B) and D)
F) All of the above

Question 9

Which of the following is true about restriction endonucleases?

Accepted Answer

A)  They make a blunt cut on the two DNA strands so that there are no single-strand regions.
B)  They make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.
C)  Some make a blunt cut on the two DNA strands so that there are no single-strand regions and some make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.
D)  Depending on the incubation conditions,the same enzyme can either make a blunt cut on the two DNA strands so that there are no single-strand regions OR make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.E) B) and C)
F) All of the above

Question 10

Which of the following is not true of cloning vectors?

Accepted Answer

A)  They usually contain multicloning sites or polylinkers.
B)  They contain at least two replication origins.
C)  They can be replicated within an appropriate host.
D)  All of these are true of cloning vectors.E) B) and C)
F) None of the above

Question 11

The Southern blotting technique for transferring DNA from an agarose gel to a piece of nitrocellulose membrane is named for
E.M.Southern,the person who developed the procedure.

Accepted Answer

A) True 
 B)False

Question 12

The most frequently chosen prokaryotic host in cloning techniques is

Accepted Answer

A)  Saccharomyces cerevisiae.
B)  Bacillus subtilis.
C)  Staphylococcus aureus.
D)  Escherichia coli.E) A) and D)
F) A) and B)

Question 13

Which of the following best describes the basis for separation of DNA fragments during agarose gel electrophoresis?

Accepted Answer

A)  The fragments with the highest percentage of G and C will migrate fastest.
B)  The fragments with the highest percentage of A and T will migrate fastest.
C)  The largest fragments will migrate fastest.
D)  The smallest fragments will migrate fastest.E) A) and B)
F) A) and C)

Question 14

The production of large quantities of a particular DNA sequence is known as gene __________________.

Accepted Answer

The answer of The production of large quantities of a...

Question 15

Some plasmid vectors have incorporated the regulatory sequences of the lactose operon so that the expression of the recombinant gene can be induced at the appropriate time.

Accepted Answer

A) True 
 B)False

Question 16

Cosmids are plasmids that can be packaged into capsids of the bacteriophage lambda; therefore,they can be transmitted like phages,but they can exist and replicate in a cell like plasmids.

Accepted Answer

A) True 
 B)False

Question 17

The PCR method was developed by

Accepted Answer

A)  Boyer.
B)  Mullis.
C)  Cohen.
D)  Sanger.E) B) and C)
F) All of the above

Question 18

Cloning a gene involves all of the following except

Accepted Answer

A)  isolating the fragment of DNA containing the desired gene.
B)  insertion of the gene into an appropriate vector.
C)  expression of the vector and the gene in a cell-free environment.
D)  introducing ligated DNA into
E)  coli cells.F) A) and E)
G) A) and B)

Question 19

The thermostable enzyme most commonly used in PCR is reverse transcriptase.

Accepted Answer

A) True 
 B)False

Question 20

Electroporation is commonly used to introduce recombinant DNA molecules into cells.

Accepted Answer

A) True 
 B)False

Exam 17: Recombinant Dna Technology

Restriction endonucleases are produced by

Correct AnswerverifiedShow Answer

Which of the following best describes the basis for separation of DNA fragments during agarose gel electrophoresis?

Correct AnswerverifiedShow Answer

___________ is a bacterial plasmid vector.

Correct AnswerverifiedShow Answer

The most frequently chosen prokaryotic host in cloning techniques is

Correct AnswerverifiedShow Answer

Which of the following is true about restriction endonucleases?

Correct AnswerverifiedShow Answer

Cosmids are plasmids that can be packaged into capsids of the bacteriophage lambda; therefore,they can be transmitted like phages,but they can exist and replicate in a cell like plasmids.

Correct AnswerverifiedShow Answer

A __________ is a DNA molecule used in hybridization reactions to detect the presence of a particular gene in separated DNA fragments.

Correct AnswerverifiedShow Answer

Some plasmid vectors have incorporated the regulatory sequences of the lactose operon so that the expression of the recombinant gene can be induced at the appropriate time.

Correct AnswerverifiedShow Answer

The production of large quantities of a particular DNA sequence is known as gene __________________.

Correct AnswerverifiedShow Answer

The PCR method was developed by

Correct AnswerverifiedShow Answer

The thermostable enzyme most commonly used in PCR is reverse transcriptase.

Correct AnswerverifiedShow Answer

Which of the following is not true of cloning vectors?

Correct AnswerverifiedShow Answer

Movement of charged molecules in an electrical field,which is used to separate nucleic acid fragments for recombinant DNA work,is called

Correct AnswerverifiedShow Answer

Electroporation is commonly used to introduce recombinant DNA molecules into cells.

Correct AnswerverifiedShow Answer

Cloning a gene involves all of the following except

Correct AnswerverifiedShow Answer

Cosmids are so named because they can be used to express foreign genes in a variety of different hosts.

Correct AnswerverifiedShow Answer

A genomic __________ is a sufficiently large collection of recombinant DNA molecules in which the inserted sequences together represent the entire genome of an organism.

Correct AnswerverifiedShow Answer

The Southern blotting technique for transferring DNA from an agarose gel to a piece of nitrocellulose membrane is named for E.M.Southern,the person who developed the procedure.

Correct AnswerverifiedShow Answer

An enzyme that cleaves internal phosphodiester bonds of a DNA molecule is a (n)

Correct AnswerverifiedShow Answer

Antibiotics incorporated into the culture medium can

Correct AnswerverifiedShow Answer

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