Question 1

The biological role of restriction enzymes is to:

Accepted Answer

A)  aid recombinant DNA research.
B)  degrade foreign DNA that enters a bacterium.
C)  make bacteria resistant to antibiotics.
D)  restrict the damage to DNA by ultraviolet light.
E)  restrict the size of DNA in certain bacteria.F) A) and B)
G) B) and E)

Question 2

Current estimates indicate that ________ % of the human genome is translated into protein.

Accepted Answer

A)  less than 0.5%
B)  roughly 1.5%
C)  roughly 10%
D)  roughly 25%
E)  more than 50%F) D) and E)
G) C) and E)

Question 3

Which of the following statements regarding plasmid-cloning vectors is correct?

Accepted Answer

A)  Circular plasmids do not require an origin of replication to be propagated in E.coli.
B)  Foreign DNA fragments up to 45,000 base pairs can be cloned in a typical plasmid.
C)  Plasmids do not need to contain genes that confer resistance to antibiotics.
D)  Plasmid vectors must carry promoters for inserted gene fragments.
E)  The copy number of plasmids may vary from a few to several hundred.F) C) and D)
G) None of the above

Question 4

The E.coli recombinant plasmid pBR322 has been widely utilized in genetic engineering experiments.pBR322 has all of the following features except:

Accepted Answer

A)  a number of conveniently located recognition sites for restriction enzymes.
B)  a number of palindromic sequences near the EcoRI site,which permit the plasmid to assume a conformation that protects newly inserted DNA from nuclease degradation.
C)  a replication origin,which permits it to replicate autonomously.
D)  resistance to two different antibiotics,which permits rapid screening for recombinant plasmids containing foreign DNA.
E)  small overall size,which facilitates entry of the plasmid into host cells.F) D) and E)
G) A) and C)

Question 5

Name one enzyme that is always used to make a cDNA library but is generally not used to make a genomic DNA library.Describe its function briefly.

Accepted Answer

Reverse transcriptase is used to make first a single-stranded DNA complementary to mRNA,then a double-stranded DNA.

Question 6

Explain how each of the following is used in cloning in a plasmid: (a)antibiotic-resistance genes
(b)origin of replication
(c)polylinker region.

Accepted Answer

(a)Antibiotic resistance allows a researcher to select for a bacterial cell clone that carries the plasmid;loss of an antibiotic marker in a strain known to contain the plasmid can be used to infer the presence of a cloned DNA segment that interrupts the antibiotic resistance gene.
(b)An origin of replication assures that the plasmid will replicate autonomously in the bacterium.
(c)Polylinkers have cut sites for a variety of restriction enzymes,allowing insertion of DNA fragments produced with any of them.

Question 7

Common features found in a cloning plasmid used for protein expression include all except which of the following?

Accepted Answer

A)  Polylinker
B)  Origin of replication.
C)  Antibiotic resistance marker(s) 
D)  Ribosome binding site
E)  Telomeric endsF) B) and D)
G) None of the above

Question 8

What is(are)the distinguishing feature(s)of a shuttle vector?

Accepted Answer

Shuttle vectors contain multip...

Question 9

A DNA sequence that may be present as only a single copy in a large mammalian genome can be amplified and cloned using the polymerase chain reaction (PCR).Describe the steps and reaction components required in a PCR experiment.Illustrate the steps in just one round.

Accepted Answer

DNA with the desired sequence is heated to convert it to single strands and cooled in the presence of an excess of oligonucleotide primers that flank the sequence to be amplified.A heat-stable DNA polymerase extends the primers,replicating the desired sequence.

Question 10

What sequences are required in an expression vector (for use with E.coli)that are not essential in a cloning plasmid?

Accepted Answer

Regulated expression of the cl...

Question 11

Which of the following methods is not used in linkage analysis?

Accepted Answer

A)  Compare densely spaced polymorphisms.
B)  Collect DNA from a family affected by the disease of interest.
C)  Sequence selected parts of the genome.
D)  Introduce retroviruses at the mutated locus.
E)  Look for SNP variants.F) A) and D)
G) All of the above

Question 12

Which of the following tags is not used to study protein function?

Accepted Answer

A)  Green fluorescent protein (GFP) 
B)  Synteny tag
C)  Tandem affinity purification (TAP) 
D)  Gal4p DNA binding domain
E)  Gal4p activation domainF) None of the above
G) B) and E)

Question 13

Which of the following statements about the polymerase chain reaction (PCR) is false?

Accepted Answer

A)  DNA amplified by PCR can be cloned.
B)  DNA amplification is linear in magnitude.
C)  Newly synthesized DNA must be heat-denatured before the next round of DNA synthesis begins.
D)  The boundaries of the amplified DNA segment are determined by the synthetic oligonucleotides used to prime DNA synthesis.
E)  The technique is sufficiently sensitive that DNA sequences can be amplified from a single animal or human hair.F) C) and E)
G) None of the above

Question 14

The size of the DNA region specifically recognized by type II restriction enzymes is typically:

Accepted Answer

A)  4 to 6 base pairs.
B)  10 to 15 base pairs.
C)  50 to 60 base pairs.
D)  200 to 300 base pairs.
E)  about the size of an average gene.F) B) and C)
G) D) and E)

Question 15

Briefly explain the procedure used in next-generation pyrosequencing.

Accepted Answer

The many DNA fragments that are to be se...

Question 16

Name two different methods by which protein-protein interactions can be discovered and probed.

Accepted Answer

1)Co-purification of protein c...

Question 17

The technique known as two hybrid analysis for detecting interacting gene products depend on:

Accepted Answer

A)  activation of DNA polymerase by the nearby binding of hybridizing protein complexes.
B)  direct binding of a Gal4p activation domain to a DNA sequence in the promoter region.
C)  having a promoter that responds directly to one of the two proteins whose interactions is being measured.
D)  hybridization of DNA segments corresponding to the two genes being examined.
E)  stimulation of transcription by interaction of two Gal4p domains via fused protein sequences.F) A) and E)
G) A) and B)

Question 18

Match between columns
                        Premises: ori sequence
BamHI sequence
tetR
PstI sequence
ampR sequence

Accepted Answer

The Answer of Insertion of foreign DNA here permits identification is...

Question 19

Current estimates indicate that humans have about ________ genes.

Accepted Answer

A)  3,000
B)  10,000
C)  30,000
D)  100,000
E)  300,000F) C) and E)
G) B) and D)

Question 20

Certain restriction enzymes produce cohesive (sticky) ends.This means that they:

Accepted Answer

A)  cut both DNA strands at the same base pair.
B)  cut in regions of high GC content,leaving ends that can form more hydrogen bonds than ends of high AT content.
C)  make a staggered double-strand cut,leaving ends with a few nucleotides of single-stranded DNA protruding.
D)  make ends that can anneal to cohesive ends generated by any other restriction enzyme.
E)  stick tightly to the ends of the DNA they have cut.F) A) and B)
G) A) and C)

Exam 9: Recombinant Dna Technology

The biological role of restriction enzymes is to:

Correct Answer
verified

Current estimates indicate that ________ % of the human genome is translated into protein.

Correct Answer
verified

Which of the following statements regarding plasmid-cloning vectors is correct?

Correct Answer
verified

The E.coli recombinant plasmid pBR322 has been widely utilized in genetic engineering experiments.pBR322 has all of the following features except:

Correct Answer
verified

Name one enzyme that is always used to make a cDNA library but is generally not used to make a genomic DNA library.Describe its function briefly.

Correct Answer
verified
Reverse transcriptase is used to make first a single-stranded DNA complementary to mRNA,then a double-stranded DNA.

Explain how each of the following is used in cloning in a plasmid: (a)antibiotic-resistance genes (b)origin of replication (c)polylinker region.

Common features found in a cloning plasmid used for protein expression include all except which of the following?

Correct Answer
verified

What is(are)the distinguishing feature(s)of a shuttle vector?

Correct Answer
verified
Shuttle vectors contain multip...
View Answer

A DNA sequence that may be present as only a single copy in a large mammalian genome can be amplified and cloned using the polymerase chain reaction (PCR).Describe the steps and reaction components required in a PCR experiment.Illustrate the steps in just one round.

Correct Answer
verified
DNA with the desired sequence is heated to convert it to single strands and cooled in the presence of an excess of oligonucleotide primers that flank the sequence to be amplified.A heat-stable DNA polymerase extends the primers,replicating the desired sequence.

What sequences are required in an expression vector (for use with E.coli)that are not essential in a cloning plasmid?

Correct Answer
verified
Regulated expression of the cl...
View Answer

Which of the following methods is not used in linkage analysis?

Correct Answer
verified

Which of the following tags is not used to study protein function?

Correct Answer
verified

Which of the following statements about the polymerase chain reaction (PCR) is false?

Correct Answer
verified

The size of the DNA region specifically recognized by type II restriction enzymes is typically:

Correct Answer
verified

Briefly explain the procedure used in next-generation pyrosequencing.

Correct Answer
verified
The many DNA fragments that are to be se...
View Answer

Name two different methods by which protein-protein interactions can be discovered and probed.

Correct Answer
verified
1)Co-purification of protein c...
View Answer

The technique known as two hybrid analysis for detecting interacting gene products depend on:

Correct Answer
verified

Match each feature of the plasmid pBR322 (at left)with one appropriate description presented (at right)(see illustration of pBR322 below).Descriptions may be used more than once.

Current estimates indicate that humans have about ________ genes.

Correct Answer
verified

Certain restriction enzymes produce cohesive (sticky) ends.This means that they:

Correct Answer
verified

Exam 9: Recombinant Dna Technology

The biological role of restriction enzymes is to:

Correct AnswerverifiedShow Answer

Current estimates indicate that ________ % of the human genome is translated into protein.

Correct AnswerverifiedShow Answer

Which of the following statements regarding plasmid-cloning vectors is correct?

Correct AnswerverifiedShow Answer

The E.coli recombinant plasmid pBR322 has been widely utilized in genetic engineering experiments.pBR322 has all of the following features except:

Correct AnswerverifiedShow Answer

Name one enzyme that is always used to make a cDNA library but is generally not used to make a genomic DNA library.Describe its function briefly.

Correct AnswerverifiedReverse transcriptase is used to make first a single-stranded DNA complementary to mRNA,then a double-stranded DNA.

Explain how each of the following is used in cloning in a plasmid: (a)antibiotic-resistance genes (b)origin of replication (c)polylinker region.

Common features found in a cloning plasmid used for protein expression include all except which of the following?

Correct AnswerverifiedShow Answer

What is(are)the distinguishing feature(s)of a shuttle vector?

Correct AnswerverifiedShuttle vectors contain multip...View AnswerShow Answer

A DNA sequence that may be present as only a single copy in a large mammalian genome can be amplified and cloned using the polymerase chain reaction (PCR).Describe the steps and reaction components required in a PCR experiment.Illustrate the steps in just one round.

Correct AnswerverifiedDNA with the desired sequence is heated to convert it to single strands and cooled in the presence of an excess of oligonucleotide primers that flank the sequence to be amplified.A heat-stable DNA polymerase extends the primers,replicating the desired sequence.

What sequences are required in an expression vector (for use with E.coli)that are not essential in a cloning plasmid?

Correct AnswerverifiedRegulated expression of the cl...View AnswerShow Answer

Which of the following methods is not used in linkage analysis?

Correct AnswerverifiedShow Answer

Which of the following tags is not used to study protein function?

Correct AnswerverifiedShow Answer

Which of the following statements about the polymerase chain reaction (PCR) is false?

Correct AnswerverifiedShow Answer

The size of the DNA region specifically recognized by type II restriction enzymes is typically:

Correct AnswerverifiedShow Answer

Briefly explain the procedure used in next-generation pyrosequencing.

Correct AnswerverifiedThe many DNA fragments that are to be se...View AnswerShow Answer

Name two different methods by which protein-protein interactions can be discovered and probed.

Correct Answerverified1)Co-purification of protein c...View AnswerShow Answer

The technique known as two hybrid analysis for detecting interacting gene products depend on:

Correct AnswerverifiedShow Answer

Match each feature of the plasmid pBR322 (at left)with one appropriate description presented (at right)(see illustration of pBR322 below).Descriptions may be used more than once.

Current estimates indicate that humans have about ________ genes.

Correct AnswerverifiedShow Answer

Certain restriction enzymes produce cohesive (sticky) ends.This means that they:

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
Reverse transcriptase is used to make first a single-stranded DNA complementary to mRNA,then a double-stranded DNA.

Correct Answer
verified

Correct Answer
verified
Shuttle vectors contain multip...
View Answer

Correct Answer
verified
DNA with the desired sequence is heated to convert it to single strands and cooled in the presence of an excess of oligonucleotide primers that flank the sequence to be amplified.A heat-stable DNA polymerase extends the primers,replicating the desired sequence.

Correct Answer
verified
Regulated expression of the cl...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
The many DNA fragments that are to be se...
View Answer

Correct Answer
verified
1)Co-purification of protein c...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified