Question 1

If the wild-type DNA sequence reads THE CAT ATE THE BIG RAT, what type of mutation would change the sequence to THE CAT ATA ETH EBI GRA T?

Accepted Answer

A)   Missense
B)   Nonsense
C)   Insertion
D)   Deletion
E)   SilentF) A) and B)
G) B) and D)

Question 2

DNA strands can be clipped crosswise at selected positions by using enzymes called ________.

Accepted Answer

A)   palindromes
B)   reverse transcriptase
C)   restriction endonucleases
D)   ligases
E)   DNA polymerasesF) A) and D)
G) B) and C)

Question 3

The process in which mutations are removed and the correct bases added is called ________.

Accepted Answer

A)   transduction
B)   excision repair
C)   frameshift
D)   back-mutation
E)   transformationF) B) and D)
G) None of the above

Question 4

Genomic ________ are collections of isolated genes maintained in a cloning host.

Accepted Answer

A)   DNA
B)   libraries
C)   clones
D)   digests
E)   booksF) D) and E)
G) A) and C)

Question 5

The RN is aware that many different types of mutations have been identified in the gene that causes cystic fibrosis. Which of the following terms would best describe a mutation that changes a normal codon into a stop codon, usually resulting in a nonfunctional protein?

Accepted Answer

A)   Missense mutation
B)   Nonsense mutation
C)   Point mutation
D)   Frameshift mutationE) A) and D)
F) A) and C)

Question 6

The antiparallel arrangement within DNA molecules refers to ________.

Accepted Answer

A)   each base bonding at the 1' position of the sugar
B)   a purine always bonding to a pyrimidine
C)   one helix strand that runs from the 5' to 3' direction and the other strand runs from the 3' to 5' direction
D)   an original parent DNA strand and one newly synthesized DNA strand comprising a new DNA molecule
E)   None of the choices are correct.F) C) and D)
G) A) and D)

Question 7

All of the following pertain to nitrogenous bases except ________.

Accepted Answer

A)   form pairs by hydrogen bonding
B)   guanine pairs with uracil
C)   adenine pairs with thymine
D)   cytosine and thymine are pyrimidines
E)   they are only present in the form of purinesF) None of the above
G) A) and B)

Question 8

Restriction endonucleases are obtained from various species of bacteria.

Accepted Answer

A) True 
 B)False

Question 9

A permanent, inheritable change in the genetic information is called ________.

Accepted Answer

A)   translation
B)   transcription
C)   mutation
D)   alteration
E)   regenerationF) B) and D)
G) None of the above

Question 10

Which of the following is not true of an organism's genotype?

Accepted Answer

A)   Is inherited
B)   Are structural genes coding for proteins
C)   Are genes coding for RNA
D)   Are regulatory genes controlling gene expression
E)   Are the expressed traits governed by the genesF) B) and E)
G) A) and D)

Question 11

When DNA is heated to 94oC, the two strands will separate.

Accepted Answer

A) True 
 B)False

Question 12

After three replication cycles in PCR, there will be a total of three double-stranded DNA molecules.

Accepted Answer

A) True 
 B)False

Question 13

The following pertain to ribosomes during protein synthesis except ________.

Accepted Answer

A)   they participate in both transcription and translation
B)   they participate only in translation
C)   they scans the mRNA for an AUG codon
D)   they are composed of ribosomal RNA and protein
E)   they shift to the right along the mRNA strand from one codon to the nextF) B) and E)
G) C) and E)

Question 14

Which tRNA anticodon would properly base-pair with an mRNA codon of cytosine-uracil-guanine (CUG) ?

Accepted Answer

A)   GTC
B)   ATC
C)   GAC
D)   UCAE) A) and D)
F) C) and D)

Question 15

A bacteriophage transfers DNA of the previous host to the current host. This is an example of ________.

Accepted Answer

A)   conjugation
B)   generalized transduction
C)   specialized transduction
D)   a reaction of an Hfr cell
E)   None of the choices are correct.F) B) and C)
G) None of the above

Question 16

The most serious type of mutation is a ________.

Accepted Answer

A)   point mutation
B)   silent mutation
C)   frameshift mutation
D)   back mutation
E)   All the choices are equally seriousF) A) and E)
G) A) and B)

Question 17

When bacteriophages invade bacterial cells, they leave behind DNA that is recognized by the bacteria as foreign and can be eliminated in the event of another attack, reminiscent of adaptive immunity. This natural process can be adapted in the lab so that a combination of guide DNA and nucleases facilitate the cutting of DNA at a precise location in any organism. Here we are describing ________.

Accepted Answer

A)   CRISPR
B)   gene therapy
C)   recombinant DNA technology
D)   synthetic biology
E)   metagenomicsF) D) and E)
G) A) and B)

Question 18

Reverse transcriptase is used to make cDNA from an RNA template.

Accepted Answer

A) True 
 B)False

Question 19

Transcription occurs in the nucleus or at the nucleoid.

Accepted Answer

A) True 
 B)False

Question 20

Among the microorganisms, various genomes can include ________.

Accepted Answer

A)   chromosomes
B)   plasmids
C)   mitochondrial DNA
D)   chloroplast DNA
E)   All of the choices are correct.F) B) and C)
G) B) and D)

Exam 8: Microbial Genetics and Genetic Engineering

The antiparallel arrangement within DNA molecules refers to ________.

Correct Answer
verified

Reverse transcriptase is used to make cDNA from an RNA template.

Correct Answer
verified

The process in which mutations are removed and the correct bases added is called ________.

Correct Answer
verified

Transcription occurs in the nucleus or at the nucleoid.

Correct Answer
verified

A bacteriophage transfers DNA of the previous host to the current host. This is an example of ________.

Correct Answer
verified

All of the following pertain to nitrogenous bases except ________.

Correct Answer
verified

A permanent, inheritable change in the genetic information is called ________.

Correct Answer
verified

Correct Answer
verified

Among the microorganisms, various genomes can include ________.

Correct Answer
verified

Which of the following is not true of an organism's genotype?

Correct Answer
verified

The following pertain to ribosomes during protein synthesis except ________.

Correct Answer
verified

After three replication cycles in PCR, there will be a total of three double-stranded DNA molecules.

Correct Answer
verified

DNA strands can be clipped crosswise at selected positions by using enzymes called ________.

Correct Answer
verified

When DNA is heated to 94^oC, the two strands will separate.

Correct Answer
verified

The RN is aware that many different types of mutations have been identified in the gene that causes cystic fibrosis. Which of the following terms would best describe a mutation that changes a normal codon into a stop codon, usually resulting in a nonfunctional protein?

Correct Answer
verified

Which tRNA anticodon would properly base-pair with an mRNA codon of cytosine-uracil-guanine (CUG) ?

Correct Answer
verified

If the wild-type DNA sequence reads THE CAT ATE THE BIG RAT, what type of mutation would change the sequence to THE CAT ATA ETH EBI GRA T?

Correct Answer
verified

Restriction endonucleases are obtained from various species of bacteria.

Correct Answer
verified

The most serious type of mutation is a ________.

Correct Answer
verified

Genomic ________ are collections of isolated genes maintained in a cloning host.

Correct Answer
verified

Exam 8: Microbial Genetics and Genetic Engineering

The antiparallel arrangement within DNA molecules refers to ________.

Correct AnswerverifiedShow Answer

Reverse transcriptase is used to make cDNA from an RNA template.

Correct AnswerverifiedShow Answer

The process in which mutations are removed and the correct bases added is called ________.

Correct AnswerverifiedShow Answer

Transcription occurs in the nucleus or at the nucleoid.

Correct AnswerverifiedShow Answer

A bacteriophage transfers DNA of the previous host to the current host. This is an example of ________.

Correct AnswerverifiedShow Answer

All of the following pertain to nitrogenous bases except ________.

Correct AnswerverifiedShow Answer

A permanent, inheritable change in the genetic information is called ________.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Among the microorganisms, various genomes can include ________.

Correct AnswerverifiedShow Answer

Which of the following is not true of an organism's genotype?

Correct AnswerverifiedShow Answer

The following pertain to ribosomes during protein synthesis except ________.

Correct AnswerverifiedShow Answer

After three replication cycles in PCR, there will be a total of three double-stranded DNA molecules.

Correct AnswerverifiedShow Answer

DNA strands can be clipped crosswise at selected positions by using enzymes called ________.

Correct AnswerverifiedShow Answer

When DNA is heated to 94oC, the two strands will separate.

Correct AnswerverifiedShow Answer

The RN is aware that many different types of mutations have been identified in the gene that causes cystic fibrosis. Which of the following terms would best describe a mutation that changes a normal codon into a stop codon, usually resulting in a nonfunctional protein?

Correct AnswerverifiedShow Answer

Which tRNA anticodon would properly base-pair with an mRNA codon of cytosine-uracil-guanine (CUG) ?

Correct AnswerverifiedShow Answer

If the wild-type DNA sequence reads THE CAT ATE THE BIG RAT, what type of mutation would change the sequence to THE CAT ATA ETH EBI GRA T?

Correct AnswerverifiedShow Answer

Restriction endonucleases are obtained from various species of bacteria.

Correct AnswerverifiedShow Answer

The most serious type of mutation is a ________.

Correct AnswerverifiedShow Answer

Genomic ________ are collections of isolated genes maintained in a cloning host.

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
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Correct Answer
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Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

When DNA is heated to 94^oC, the two strands will separate.

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified