Question 1

It was not until the discovery of ________ that organ and tissue culture became feasible.

Accepted Answer

A)  hydroponics
B)  plant hormones
C)  the importance of sucrose in nutrient solutions
D)  the essential mineral elements
E)  biotechnologyF) A) and B)
G) B) and E)

Question 2

The preparation of knockout mutants involves:

Accepted Answer

A)  inserting a piece of DNA into the gene of interest.
B)  the use of closely packed DNA fragments attached to a glass slide.
C)  searching for homology.
D)  determining the number of base pairs in a gene.
E)  determining the rate of recombination.F) A) and B)
G) A) and D)

Question 3

A sticky-ended fragment can join with another DNA segment only if that segment:

Accepted Answer

A)  has been cut with the same restriction enzyme.
B)  has been cut with the same ligase.
C)  is palindromic.
D)  is part of a plasmid.
E)  is part of a virus.F) A) and B)
G) A) and C)

Question 4

By definition, a transformed cell:

Accepted Answer

A)  contains a plasmid.
B)  contains a recombinant DNA molecule.
C)  contains a virus.
D)  functions as a vector.
E)  divides in such a way so as to amplify the DNA fragment.F) B) and D)
G) C) and D)

Question 5

"Golden rice" is notable because of its:

Accepted Answer

A)  high β-carotene content.
B)  high starch content.
C)  resistance to herbicides.
D)  delayed senescence.
E)  resistance to insect larvae.F) D) and E)
G) All of the above

Question 6

Sticky-ended fragments can be joined by specific restriction enzymes.

Accepted Answer

A) True 
 B)False

Question 7

Which of the following statements about eukaryotic genomes is FALSE?

Accepted Answer

A)  They are larger than those of prokaryotes.
B)  In general, multicellular eukaryotes have more DNA than unicellular eukaryotes.
C)  The greater the complexity of the organism, the more protein-coding genes it has.
D)  The roundworm Caenorhacditis has more genes than the fruit fly Drosophila.
E)  The plant Arabidopsis has more genes than the fruit fly Drosophila.F) A) and D)
G) A) and C)

Question 8

Particle bombardment and electroporation are the two techniques most widely used to transfer DNA into plants.

Accepted Answer

A) True 
 B)False

Question 9

The ampR gene is used in recombinant DNA technology to:

Accepted Answer

A)  produce β-galactosidase.
B)  clone a particular gene fragment.
C)  screen for the presence of cells containing recombinant plasmids.
D)  help the bacterium to survive on a medium containing lactose.
E)  transform a bacterial cell.F) None of the above
G) B) and E)

Question 10

The first eukaryotic genome to be sequenced was that of:

Accepted Answer

A)  Haemophilus influenzae.
B)  Saccharomyces cerevisae.
C)  Arabidopsis thaliana.
D)  Agrobacterium tumefaciens.
E)  Caenorhabditis elegans.F) A) and B)
G) C) and E)

Question 11

Which of the following statements concerning restriction enzymes is FALSE?

Accepted Answer

A)  They are a type of endonuclease.
B)  They recognize DNA sequences typically four to six nucleotides long.
C)  They recognize single-stranded DNA sequences.
D)  Some make straight cuts in the DNA.
E)  They recognize specific palindromic sequences.F) A) and C)
G) B) and E)

Question 12

A search for homology would be used to determine the possible function of a gene.

Accepted Answer

A) True 
 B)False

Question 13

DNA fragments having sticky ends can join with other fragments obtained by using the same restriction enzyme.

Accepted Answer

A) True 
 B)False

Question 14

By definition, hydroponics is the technique for growing:

Accepted Answer

A)  shoot tips.
B)  isolated leaf pieces.
C)  isolated roots
D)  plant embryos.
E)  plants without soil.F) B) and C)
G) A) and C)

Question 15

Agrobacterium tumefaciens receives the Ti plasmid from its host plant.

Accepted Answer

A) True 
 B)False

Question 16

Mutant forms of the ethylene receptor gene from Arabidopsis are inserted into plants for the purpose of:

Accepted Answer

A)  increasing starch content.
B)  increasing levels of β-carotene.
C)  stimulating fruit ripening.
D)  conferring resistance to pesticides.
E)  delaying flower wilting.F) B) and E)
G) B) and C)

Question 17

In Agrobacterium, genes that code for enzymes involved in the synthesis of the hormone auxin are found in the _____ region of the plasmid.

Accepted Answer

A)  R
B)  O
C)  onc
D)  vir
E)  AF) A) and B)
G) A) and E)

Question 18

Identical DNA fragments are obtained by cutting the same DNA molecule with different restriction enzymes.

Accepted Answer

A) True 
 B)False

Question 19

The Ti plasmid of Agrobacterium tumefaciens contains genes for plant hormones as well as genes for molecules that nourish the bacterium.

Accepted Answer

A) True 
 B)False

Question 20

An example of a selectable marker gene is:

Accepted Answer

A)  the gene for a green fluorescent protein.
B)  the luciferase gene.
C)  EcoRI.
D)  lacZ.
E)  ampR.F) B) and D)
G) None of the above

Exam 10: Recombinant Dna Technology, Plant Biotechnology, and Genomics

Mutant forms of the ethylene receptor gene from Arabidopsis are inserted into plants for the purpose of:

Correct AnswerverifiedShow Answer

Agrobacterium tumefaciens receives the Ti plasmid from its host plant.

Correct AnswerverifiedShow Answer

A sticky-ended fragment can join with another DNA segment only if that segment:

Correct AnswerverifiedShow Answer

It was not until the discovery of ________ that organ and tissue culture became feasible.

Correct AnswerverifiedShow Answer

In Agrobacterium, genes that code for enzymes involved in the synthesis of the hormone auxin are found in the _____ region of the plasmid.

Correct AnswerverifiedShow Answer

DNA fragments having sticky ends can join with other fragments obtained by using the same restriction enzyme.

Correct AnswerverifiedShow Answer

Which of the following statements concerning restriction enzymes is FALSE?

Correct AnswerverifiedShow Answer

A search for homology would be used to determine the possible function of a gene.

Correct AnswerverifiedShow Answer

The Ti plasmid of Agrobacterium tumefaciens contains genes for plant hormones as well as genes for molecules that nourish the bacterium.

Correct AnswerverifiedShow Answer

The ampR gene is used in recombinant DNA technology to:

Correct AnswerverifiedShow Answer

Particle bombardment and electroporation are the two techniques most widely used to transfer DNA into plants.

Correct AnswerverifiedShow Answer

The preparation of knockout mutants involves:

Correct AnswerverifiedShow Answer

By definition, a transformed cell:

Correct AnswerverifiedShow Answer

Which of the following statements about eukaryotic genomes is FALSE?

Correct AnswerverifiedShow Answer

Identical DNA fragments are obtained by cutting the same DNA molecule with different restriction enzymes.

Correct AnswerverifiedShow Answer

An example of a selectable marker gene is:

Correct AnswerverifiedShow Answer

Sticky-ended fragments can be joined by specific restriction enzymes.

Correct AnswerverifiedShow Answer

"Golden rice" is notable because of its:

Correct AnswerverifiedShow Answer

By definition, hydroponics is the technique for growing:

Correct AnswerverifiedShow Answer

The first eukaryotic genome to be sequenced was that of:

Correct AnswerverifiedShow Answer

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The amp^R gene is used in recombinant DNA technology to:

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