Question 1

Which enzyme elongates the new DNA strand starting at an RNA primer?

Accepted Answer

A)   DNA polymerase III
B)   DNA ligase
C)   RNA primase
D)   RNA polymerase
E)   DNA polymerase IF) C) and D)
G) A) and E)

Question 2

DNA primase is an enzyme that catalyzes the reaction necessary to synthesize a molecule of DNA.

Accepted Answer

A) True 
 B)False  False

Question 3

The diploid set of chromosomes in Drosophila embryos replicates six times faster than the single E. coli chromosome, even though there is about 100 times more DNA in Drosophila than in E. coli and the rate of movement of the replication fork in Drosophila is much slower. How is this so?

Accepted Answer

Eukaryotic chromosomes duplicate rapidly...

Question 4

At the growing end of a DNA chain, DNA polymerase catalyzes the formation of a disulfide bond between the 3'-OH group of the deoxyribose on the last nucleotide and the 5'-phosphate of the dNTP precursor.

Accepted Answer

A) True 
 B)False

Question 5

What is a method by which you could visualize semiconservative DNA replication in eukaryotes?

Accepted Answer

You could use chromosomal staining in ce...

Question 6

Topoisomerase and SSB proteins are important components of the replication process in prokaryotes, but they are not found in eukaryotes.

Accepted Answer

A) True 
 B)False

Question 7

Which of the following are necessary for DNA replication in vitro?

Accepted Answer

A)   DNA can't replicate in vitro
B)   Okazaki fragments, helicase, DNA polymerase
C)   RNA, helicase, DNA polymerase
D)   Template DNA, DNA polymerase, four dNTPs, magnesium ions
E)   Template DNA, four dNTPs, and magnesium ionsF) A) and E)
G) A) and D)

Question 8

When the RNA primers are removed from the 5' ends of eukaryotic chromosomes after replication, DNA polymerase is unable to fill in the gaps. What prevents the chromosomes from getting shorter and shorter with each round of replication?

Accepted Answer

The enzyme telomerase maintains chromoso...

Question 9

Only the leading strand of a DNA molecule serves as a template during replication.

Accepted Answer

A) True 
 B)False

Question 10

In eukaryotic cells, RNA and proteins are actively synthesized during the S phase of the cell cycle.

Accepted Answer

A) True 
 B)False

Question 11

What are the key replication enzymes at the replisome, and how is DNA replication on both leading and lagging strands made efficient through the conformation of the DNA at the replisome?

Accepted Answer

The key replication enzymes at the replisome are helicase, primase, and DNA polymerase III. To make replication more efficient, the lagging-strand DNA is folded so that its DNA polymerase III is complexed with the DNA polymerase III on the leading strand (forming the DNA Pol III holoenzyme). The folding of the lagging-strand template also makes production of sequential Okazaki fragments more efficient by bringing the 3' end of each completed Okazaki fragments near the site where the next Okazaki fragment will start.

Question 12

In both prokaryotes and eukaryotes, DNA replication is

Accepted Answer

A)   bidirectional and semiconservative.
B)   bidirectional and dispersive.
C)   unidirectional and dispersive.
D)   dispersive and semiconservative.
E)   unidirectional and semiconservative.F) B) and E)
G) A) and B)

Question 13

Which enzyme activity is associated with DNA polymerase I?

Accepted Answer

A)   5' to 3' polymerase
B)   3' to 5' exonuclease
C)   5' to 3' exonuclease
D)   Both A and B
E)   All of the aboveF) A) and D)
G) A) and E)

Question 14

Which kind of enzyme prevents DNA from tangling up by introducing negative supercoils as the replication fork migrates during replication?

Accepted Answer

A)   Helicase
B)   DNA polymerase I
C)   Topoisomerase
D)   DNA polymerase III
E)   LigaseF) A) and D)
G) C) and D)

Question 15

In eukaryotes, DNA replication begins at a single origin of replication on each chromosome.

Accepted Answer

A) True 
 B)False

Question 16

After removal of the RNA primers and replacement with DNA nucleotides, the single-stranded nick adjacent to Okazaki fragments is filled in through a reaction that involves which enzyme?

Accepted Answer

A)   DNA ligase
B)   SSB protein
C)   DNA primase
D)   RNA polymerase
E)   DNA helicaseF) C) and D)
G) B) and D)

Question 17

The enzymatic activity of a telomerase is best described as a

Accepted Answer

A)   polymerase.
B)   reverse transcriptase.
C)   exonuclease.
D)   ligase.
E)   topoisomerase.F) A) and E)
G) B) and E) B

Question 18

The two most basic steps of DNA replication are

Accepted Answer

A)   the new strand is denatured and a template is synthesized.
B)   helicase unwinds template and DNA polymerase binds.
C)   primase causes primer to bind template and ligase copies the template.
D)   the template is denatured and a new strand is synthesized.
E)   leading strand is copied first and lagging strand second.F) B) and D)
G) A) and B)

Question 19

As helicase unwinds the DNA molecule, what keeps the strands apart?

Accepted Answer

A)   Replication fork
B)   Okazaki fragments
C)   DNA polymerase
D)   Reverse transcriptase
E)   Single-strand binding proteinsF) A) and E)
G) None of the above

Question 20

Where does the initiator protein bind DNA at the start of replication?

Accepted Answer

A)   At a replication fork
B)   At an origin of replication
C)   At a promoter region
D)   At a start codon
E)   At any AT-rich regionF) A) and D)
G) D) and E)

Exam 10: Non-Mendelian Inheritance

Which enzyme elongates the new DNA strand starting at an RNA primer?

Correct Answer
verified

DNA primase is an enzyme that catalyzes the reaction necessary to synthesize a molecule of DNA.

Correct Answer
verified
False

The diploid set of chromosomes in Drosophila embryos replicates six times faster than the single E. coli chromosome, even though there is about 100 times more DNA in Drosophila than in E. coli and the rate of movement of the replication fork in Drosophila is much slower. How is this so?

Correct Answer
verified
Eukaryotic chromosomes duplicate rapidly...
View Answer

At the growing end of a DNA chain, DNA polymerase catalyzes the formation of a disulfide bond between the 3'-OH group of the deoxyribose on the last nucleotide and the 5'-phosphate of the dNTP precursor.

Correct Answer
verified

What is a method by which you could visualize semiconservative DNA replication in eukaryotes?

Correct Answer
verified
You could use chromosomal staining in ce...
View Answer

Topoisomerase and SSB proteins are important components of the replication process in prokaryotes, but they are not found in eukaryotes.

Correct Answer
verified

Which of the following are necessary for DNA replication in vitro?

Correct Answer
verified

When the RNA primers are removed from the 5' ends of eukaryotic chromosomes after replication, DNA polymerase is unable to fill in the gaps. What prevents the chromosomes from getting shorter and shorter with each round of replication?

Correct Answer
verified
The enzyme telomerase maintains chromoso...
View Answer

Only the leading strand of a DNA molecule serves as a template during replication.

Correct Answer
verified

In eukaryotic cells, RNA and proteins are actively synthesized during the S phase of the cell cycle.

Correct Answer
verified

What are the key replication enzymes at the replisome, and how is DNA replication on both leading and lagging strands made efficient through the conformation of the DNA at the replisome?

In both prokaryotes and eukaryotes, DNA replication is

Correct Answer
verified

Which enzyme activity is associated with DNA polymerase I?

Correct Answer
verified

Which kind of enzyme prevents DNA from tangling up by introducing negative supercoils as the replication fork migrates during replication?

Correct Answer
verified

In eukaryotes, DNA replication begins at a single origin of replication on each chromosome.

Correct Answer
verified

After removal of the RNA primers and replacement with DNA nucleotides, the single-stranded nick adjacent to Okazaki fragments is filled in through a reaction that involves which enzyme?

Correct Answer
verified

The enzymatic activity of a telomerase is best described as a

Correct Answer
verified
B

The two most basic steps of DNA replication are

Correct Answer
verified

As helicase unwinds the DNA molecule, what keeps the strands apart?

Correct Answer
verified

Where does the initiator protein bind DNA at the start of replication?

Correct Answer
verified

Exam 10: Non-Mendelian Inheritance

Which enzyme elongates the new DNA strand starting at an RNA primer?

Correct AnswerverifiedShow Answer

DNA primase is an enzyme that catalyzes the reaction necessary to synthesize a molecule of DNA.

Correct AnswerverifiedFalse

The diploid set of chromosomes in Drosophila embryos replicates six times faster than the single E. coli chromosome, even though there is about 100 times more DNA in Drosophila than in E. coli and the rate of movement of the replication fork in Drosophila is much slower. How is this so?

Correct AnswerverifiedEukaryotic chromosomes duplicate rapidly...View AnswerShow Answer

At the growing end of a DNA chain, DNA polymerase catalyzes the formation of a disulfide bond between the 3'-OH group of the deoxyribose on the last nucleotide and the 5'-phosphate of the dNTP precursor.

Correct AnswerverifiedShow Answer

What is a method by which you could visualize semiconservative DNA replication in eukaryotes?

Correct AnswerverifiedYou could use chromosomal staining in ce...View AnswerShow Answer

Topoisomerase and SSB proteins are important components of the replication process in prokaryotes, but they are not found in eukaryotes.

Correct AnswerverifiedShow Answer

Which of the following are necessary for DNA replication in vitro?

Correct AnswerverifiedShow Answer

When the RNA primers are removed from the 5' ends of eukaryotic chromosomes after replication, DNA polymerase is unable to fill in the gaps. What prevents the chromosomes from getting shorter and shorter with each round of replication?

Correct AnswerverifiedThe enzyme telomerase maintains chromoso...View AnswerShow Answer

Only the leading strand of a DNA molecule serves as a template during replication.

Correct AnswerverifiedShow Answer

In eukaryotic cells, RNA and proteins are actively synthesized during the S phase of the cell cycle.

Correct AnswerverifiedShow Answer

What are the key replication enzymes at the replisome, and how is DNA replication on both leading and lagging strands made efficient through the conformation of the DNA at the replisome?

In both prokaryotes and eukaryotes, DNA replication is

Correct AnswerverifiedShow Answer

Which enzyme activity is associated with DNA polymerase I?

Correct AnswerverifiedShow Answer

Which kind of enzyme prevents DNA from tangling up by introducing negative supercoils as the replication fork migrates during replication?

Correct AnswerverifiedShow Answer

In eukaryotes, DNA replication begins at a single origin of replication on each chromosome.

Correct AnswerverifiedShow Answer

After removal of the RNA primers and replacement with DNA nucleotides, the single-stranded nick adjacent to Okazaki fragments is filled in through a reaction that involves which enzyme?

Correct AnswerverifiedShow Answer

The enzymatic activity of a telomerase is best described as a

Correct AnswerverifiedB

The two most basic steps of DNA replication are

Correct AnswerverifiedShow Answer

As helicase unwinds the DNA molecule, what keeps the strands apart?

Correct AnswerverifiedShow Answer

Where does the initiator protein bind DNA at the start of replication?

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified
False

Correct Answer
verified
Eukaryotic chromosomes duplicate rapidly...
View Answer

Correct Answer
verified

Correct Answer
verified
You could use chromosomal staining in ce...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
The enzyme telomerase maintains chromoso...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
B

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified