Question 1

Properties of cloning vectors include each of the following, except

Accepted Answer

A)   they may contain sequences which allow them to be replicated autonomously within a compatible host cell.
B)   they may contain sequences which enable integration into the host genome.
C)   only a select few of them may have a unique cloning site which can be cut by restriction endonucleases.
D)   the most important vectors have restriction sites which are grouped together in a polylinker.E) All of the above
F) None of the above

Question 2

To be useful as a vector, restriction enzyme reaction sites should be placed

Accepted Answer

A)   around antibiotic resistance genes.
B)   within antibiotic resistance genes.
C)   in the ori site.
D)   inside TRP1 or URA3 genes.E) None of the above
F) B) and D)

Question 3

DNA molecules which are constructed with DNA from different sources are called

Accepted Answer

A)   exons.
B)   introns.
C)   transgenic DNA molecules.
D)   recombinant DNA molecules.E) C) and D)
F) None of the above

Question 4

Insulin and growth hormones are two examples of proteins that can be produced in great quantities by their synthesis in bacteria.

Accepted Answer

A) True 
 B)False

Question 5

was the first bacterial genome sequence to be published.

Accepted Answer

A)   Haemophilus influenzae
B)   E. coli
C)   Agrobacterium tumefaciens
D)   S. cerevisiaeE) All of the above
F) B) and C)

Question 6

Which isare)  appropriate for use as a labeled probes)  to screen a DNA library?

Accepted Answer

A)   cloned cDNA
B)   antibodies
C)   mRNA
D)   All of the aboveE) A) and D)
F) B) and C)

Question 7

Which of the following is false?

Accepted Answer

A)   Blunt end fragments of DNA can be ligated.
B)   A sheared DNA fragment can be given sticky ends.
C)   The enzyme deoxynucleotidyl transferase cannot generate sticky ends.
D)   A synthetic DNA fragment, called a linker, can connect blunt end DNA fragments.E) None of the above
F) A) and D)

Question 8

Complementary DNA cDNA is single-stranded DNA made with reverse transcriptase.

Accepted Answer

A) True 
 B)False

Question 9

What is the frequency of occurrence of a desired clone from a YAC library of genomic DNA if the insert size is 400 kbp and the genome contains 2 × 106 kbp?

Accepted Answer

A)   2 × 10-4 
B)   8 × 108 
C)   5 × 103 
D)   1.25 × 10-9E) A) and B)
F) A) and C)

Question 10

Reverse transcriptase is a vital enzyme in the development of a genomic DNA library.

Accepted Answer

A) True 
 B)False

Question 11

Insert DNA may be produced by

Accepted Answer

A)   the mechanical shearing of long DNA molecules.
B)   digesting DNA with type II restriction endonucleases.
C)   the action of reverse transcriptase on rRNA.
D)   All of the above
E)   Only A and BF) All of the above
G) C) and D)

Question 12

is the direct uptake of foreign DNA by a host cell.

Accepted Answer

A)   Transfection
B)   Transduction
C)   Transformation
D)   ConjugationE) A) and B)
F) A) and C)

Question 13

Which is not necessary as part of an expression vector used in E. coli?

Accepted Answer

A)   marker gene
B)   ribosome binding site
C)   Taq polymerase gene
D)   strong promoter sequenceE) A) and B)
F) None of the above

Question 14

The human genome contains about bp of DNA.

Accepted Answer

A)   3.2 thousand
B)   3.2 million
C)   3.2 billion
D)   3.2 trillionE) A) and B)
F) All of the above

Question 15

How might infection of a plant by E. coli transformed with a recombinant Ti plasmid be used to have a new gene e.g. insect toxin)  expressed in all cells of the plant?

Accepted Answer

A)   The original plant will eventually express the new gene in all its cells even if only a few cells of the plant are initially infected by E. coli.
B)   Pollen cells must be infected and then used to produce the next generation of plants which will express the new gene in all cells.
C)   Any cell from the original plant that is infected with the E. coli is removed and treated to dedifferentiate and eventually to grow into an entire new plant in which the new gene is expressed throughout.
D)   All of the aboveE) A) and B)
F) B) and D)

Question 16

is the propagation of individual host cells containing a recombinant DNA molecule.

Accepted Answer

A)   Transformation
B)   Cloning
C)   Transduction
D)   None of the aboveE) None of the above
F) C) and D)

Question 17

Which of the following is not an application of PCR?

Accepted Answer

A)   formation of proteins from RNA fragments
B)   investigation of molecular evolution
C)   amplification of genes in paternity testing
D)   construction of phylogenetic trees
E)   detection of microoranisms from clinical specimens blood, urine, etc.) F) C) and D)
G) A) and E)

Question 18

General steps in the generation of a recombinant DNA molecule in the laboratory include

Accepted Answer

A)   ligation of DNA segments.
B)   cleavage of DNA into small fragments.
C)   insertion of DNA fragments into a vector.
D)   All of the aboveE) None of the above
F) A) and B)

Question 19

Under normal conditions, about what minimum range of sequence similarity is desired to obtain hybridization between a cDNA probe and the desired gene in a library?

Accepted Answer

A)   5% to 10%
B)   30% to 40%
C)   60% to 70%
D)   90% to 95%E) A) and D)
F) A) and C)

Question 20

How many recombinant clones must be screened in order to be 99% certain that a particular gene is found in a YAC library of human DNA where the average size of an insert is 500 kbp? The human genome size is about 3.2 × 106 kbp.

Accepted Answer

A)   6000
B)   30,000
C)   1,000,000
D)   30,000,000E) A) and D)
F) A) and B)

Exam 23: Recombinant Dna Technology

How many recombinant clones must be screened in order to be 99% certain that a particular gene is found in a YAC library of human DNA where the average size of an insert is 500 kbp? The human genome size is about 3.2 × 106 kbp.

Correct AnswerverifiedShow Answer

is the direct uptake of foreign DNA by a host cell.

Correct AnswerverifiedShow Answer

What is the frequency of occurrence of a desired clone from a YAC library of genomic DNA if the insert size is 400 kbp and the genome contains 2 × 106 kbp?

Correct AnswerverifiedShow Answer

Under normal conditions, about what minimum range of sequence similarity is desired to obtain hybridization between a cDNA probe and the desired gene in a library?

Correct AnswerverifiedShow Answer

General steps in the generation of a recombinant DNA molecule in the laboratory include

Correct AnswerverifiedShow Answer

The human genome contains about bp of DNA.

Correct AnswerverifiedShow Answer

To be useful as a vector, restriction enzyme reaction sites should be placed

Correct AnswerverifiedShow Answer

is the propagation of individual host cells containing a recombinant DNA molecule.

Correct AnswerverifiedShow Answer

Properties of cloning vectors include each of the following, except

Correct AnswerverifiedShow Answer

Insulin and growth hormones are two examples of proteins that can be produced in great quantities by their synthesis in bacteria.

Correct AnswerverifiedShow Answer

Which of the following is false?

Correct AnswerverifiedShow Answer

was the first bacterial genome sequence to be published.

Correct AnswerverifiedShow Answer

Insert DNA may be produced by

Correct AnswerverifiedShow Answer

Which isare) appropriate for use as a labeled probes) to screen a DNA library?

Correct AnswerverifiedShow Answer

DNA molecules which are constructed with DNA from different sources are called

Correct AnswerverifiedShow Answer

Which of the following is not an application of PCR?

Correct AnswerverifiedShow Answer

Which is not necessary as part of an expression vector used in E. coli?

Correct AnswerverifiedShow Answer

How might infection of a plant by E. coli transformed with a recombinant Ti plasmid be used to have a new gene e.g. insect toxin) expressed in all cells of the plant?

Correct AnswerverifiedShow Answer

Reverse transcriptase is a vital enzyme in the development of a genomic DNA library.

Correct AnswerverifiedShow Answer

Complementary DNA cDNA is single-stranded DNA made with reverse transcriptase.

Correct AnswerverifiedShow Answer

How many recombinant clones must be screened in order to be 99% certain that a particular gene is found in a YAC library of human DNA where the average size of an insert is 500 kbp? The human genome size is about 3.2 × 10⁶kbp.

Correct Answer
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What is the frequency of occurrence of a desired clone from a YAC library of genomic DNA if the insert size is 400 kbp and the genome contains 2 × 10⁶kbp?

Correct Answer
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