Question 1

What aspect of a common recombinant DNA technology tool makes direct use of knowledge acquired from studies of retrovirus replication?

Accepted Answer

A)   Using restriction endonucleases to cleave DNA
B)   Complementary DNA (cDN
A)   synthesis
C)   Introducing DNA into bacterial cells by transformation
D)   Using DNA ligase to join two pieces of DNAE) None of the above
F) A) and B)

Question 2

To prepare a hybridization probe for screening a library, which labeling technique would be most desirable?

Accepted Answer

A)   Klenow fill-in
B)   5 ' end-labeling
C)   random primed labeling
D)   3 'end-labelingE) A) and C)
F) A) and D)

Question 3

Following the injection of a linear phage DNA into E. coli host cells, the phage genome circularizes by

Accepted Answer

A)   recombination at attachment (Att)  sites.
B)   joining of the attachment (Att)  sites.
C)   recombination at cohesive (cos)  sites.
D)   joining of the cohesive (cos)  sites.E) None of the above
F) C) and D)

Question 4

The appropriate hybridization temperature during library screening is calculated according to the G + C content and the percent homology of the probe to target. The more mismatches there are:

Accepted Answer

A)   the higher the melting temperature (Tm)  will be.
B)   the lower the melting temperature (Tm)  will be.
C)   the more stable the heteroduplex will be.
D)   the more stringent the wash should be.E) A) and C)
F) A) and B)

Question 5

Explain the terms "random walk" and "coupling" in the context of the mode of action of restriction endonucleases.

Accepted Answer

Random walk refers to the movement of a ...

Question 6

Which statement is not true about DNA binding and cleavage by type II restriction endonucleases?

Accepted Answer

A)   the recognition process triggers a conformational change in both the enzyme and the DNA.
B)   the first contact with DNA is specific.
C)   the target site is located by sliding, hopping, and jumping.
D)   the enzyme interacts directly with the nitrogenous bases.E) B) and C)
F) All of the above

Question 7

You want to join together two pieces of DNA that have been cut with SmaI. Outline a strategy you could use to increase the efficiency of blunt end ligation.

Accepted Answer

One strategy to increase the efficiency ...

Question 8

In a single PCR cycle consisting of 30 seconds at 95C, 1 min at 55C, and 1 min at 72C, what is happening in the step run at 55C?

Accepted Answer

A)   The DNA to be amplified is being denatured.
B)   Primers are being denatured.
C)   DNA polymerase is extending new DNA from the primers.
D)   Primers are annealing to the DNA to be amplified.E) A) and B)
F) C) and D)

Question 9

You plan to use the polymerase chain reaction to amplify part of the DNA sequence shown below, using oligonucleotide primers that are hexamers matching the regions shown in red. (In practice, hexamers are too short for most purposes). State the sequence of the primer oligonucleotides that should be used, including their polarity (5′3′), and give the sequence of the DNA molecule that results from amplification.
5′-TAGGCATGCAATGGTAATTTTTCAGGAACCAGGGCCCTTAAGCCGTAGGCAT-3′
3′-ATCGGTACGTTACCATTAAAAACTCCTTGGTCCCGGGAATTCGGCATCGGTA-5′

Accepted Answer

Primers: 5'-CGGCTT-3' and 5'-G...

Question 10

You want to make a genomic library with DNA fragments averaging about 85 kb in length. Which vectors discussed would be appropriate to use? Which would be inappropriate? Explain your answer.

Accepted Answer

To create a genomic library with DNA fra...

Question 11

How can an amino acid sequence be used to design a gene-specific hybridization probe?

Accepted Answer

A)   Synthesize a portion of a polypeptide chain based on the amino acid sequence of the full protein, and use this polypeptide chain as a probe.
B)   Purify a protein that contains this amino acid sequence, digest it with proteases that cleave at specific sites, and use one of these peptide fragments as a probe.
C)   Synthesize all possible nucleotide sequences long enough to encode the amino acid sequence and thereby find one sequence that will encode the amino acid sequence.
D)   Synthesize a set of nucleotide sequences that could encode the amino acid sequence, and this set of oligonucleotides as a probe.E) C) and D)
F) All of the above

Question 12

What is the difference between a restriction endonuclease recognition site and a RFLP?

Accepted Answer

A)   Restriction endonuclease recognition sites are any sequence of DNA recognized by a restriction endonuclease; RFLPs are any sequence of RNA amplified by reverse transcriptase and PCR.
B)   Restriction endonuclease recognition sites produce DNA fragments that differ in size when the source of the DNA is from different chromosomes; RFLPs are any sequence of RNA amplified by reverse transcriptase and PCR.
C)   Restriction endonuclease recognition sites produce DNA fragments that differ in size when the source of the DNA is from different chromosomes; RFLPs are any sequence of DNA recognized by a restriction endonuclease.
D)   Restriction endonuclease recognition sites are any sequence of DNA recognized by a restriction endonuclease; RFLPs are DNA fragments produced by restriction endonuclease digestion that differ in size when DNA sequences differ between chromosomes or individuals.E) B) and D)
F) C) and D)

Question 13

To read an old-fashioned Sanger sequencing gel in the 5' to 3' direction, on must read the gel

Accepted Answer

A)   from top to bottom.
B)   from bottom to top.
C)   from left to right.
D)   from right to left.E) A) and D)
F) A) and C)

Question 14

Explain why DNA polymerase from a thermophilic bacterium is used in PCR instead of DNA polymerase I from E. coli.

Accepted Answer

DNA polymerase from a thermophilic bacte...

Question 15

You want to clone a 1.5 kb cDNA. Which vectors discussed would be appropriate to use? Which would be inappropriate? Explain your answer.

Accepted Answer

The appropriate vectors to use for cloni...

Question 16

What is suggested by the fact that type II restriction endonuclease recognition sequences are usually palindromic?

Accepted Answer

A)   A particular restriction endonuclease can cleave DNA at several different sites.
B)   Restriction endonucleases must cleave DNA in a multistep process.
C)   Restriction endonucleases function as dimers.
D)   Restriction endonucleases recognize the sugar-phosphate backbone of DNA.E) A) and D)
F) A) and C)

Question 17

Which step in Southern blotting allows an investigator to identify a particular DNA fragment that is present within a complex mixture of fragments?

Accepted Answer

A)   restriction endonuclease digestion of DNA
B)   agarose gel electrophoresis
C)   blotting
D)   hybridization with a labeled probeE) A) and D)
F) A) and B)

Question 18

What does it mean when some bands on an autoradiograph are darker than others?

Accepted Answer

A)   The concentration of the labeled macromolecule in that band is higher.
B)   The concentration of the labeled macromolecule in that band is lower.
C)   The experiment failed because the bands should be of the same intensity throughout the autoradiograph.
D)   The darker bands represent larger labeled macromolecules.E) C) and D)
F) B) and D)

Question 19

Which of the following vectors can accommodate the largest DNA insert?

Accepted Answer

A)   plasmid
B)   cosmid
C)   phage
D)   pUC18E) A) and B)
F) C) and D)

Question 20

Describe the principle of ion-exchange chromatography. Describe the behavior of a positively charged protein and a negatively charged protein in a column containing positively charged resin during sample loading and elution.

Accepted Answer

Ion-exchange chromatography is a techniq...

Exam 8: Recombinant Dna Technology and Molecular Cloning

What is suggested by the fact that type II restriction endonuclease recognition sequences are usually palindromic?

Correct Answer
verified

To prepare a hybridization probe for screening a library, which labeling technique would be most desirable?

Correct Answer
verified

Following the injection of a linear phage DNA into E. coli host cells, the phage genome circularizes by

Correct Answer
verified

Explain the terms "random walk" and "coupling" in the context of the mode of action of restriction endonucleases.

Correct Answer
Answered by ExamLex AI
Random walk refers to the movement of a ...
View Answer

You want to join together two pieces of DNA that have been cut with SmaI. Outline a strategy you could use to increase the efficiency of blunt end ligation.

Correct Answer
Answered by ExamLex AI
One strategy to increase the efficiency ...
View Answer

Describe the principle of ion-exchange chromatography. Describe the behavior of a positively charged protein and a negatively charged protein in a column containing positively charged resin during sample loading and elution.

Correct Answer
Answered by ExamLex AI
Ion-exchange chromatography is a techniq...
View Answer

To read an old-fashioned Sanger sequencing gel in the 5' to 3' direction, on must read the gel

Correct Answer
verified

You want to make a genomic library with DNA fragments averaging about 85 kb in length. Which vectors discussed would be appropriate to use? Which would be inappropriate? Explain your answer.

Correct Answer
Answered by ExamLex AI
To create a genomic library with DNA fra...
View Answer

Explain why DNA polymerase from a thermophilic bacterium is used in PCR instead of DNA polymerase I from E. coli.

Correct Answer
Answered by ExamLex AI
DNA polymerase from a thermophilic bacte...
View Answer

Which statement is not true about DNA binding and cleavage by type II restriction endonucleases?

Correct Answer
verified

You want to clone a 1.5 kb cDNA. Which vectors discussed would be appropriate to use? Which would be inappropriate? Explain your answer.

Correct Answer
Answered by ExamLex AI
The appropriate vectors to use for cloni...
View Answer

What aspect of a common recombinant DNA technology tool makes direct use of knowledge acquired from studies of retrovirus replication?

Correct Answer
verified

How can an amino acid sequence be used to design a gene-specific hybridization probe?

Correct Answer
verified

What does it mean when some bands on an autoradiograph are darker than others?

Correct Answer
verified

The appropriate hybridization temperature during library screening is calculated according to the G + C content and the percent homology of the probe to target. The more mismatches there are:

Correct Answer
verified

In a single PCR cycle consisting of 30 seconds at 95C, 1 min at 55C, and 1 min at 72C, what is happening in the step run at 55C?

Correct Answer
verified

Which step in Southern blotting allows an investigator to identify a particular DNA fragment that is present within a complex mixture of fragments?

Correct Answer
verified

What is the difference between a restriction endonuclease recognition site and a RFLP?

Correct Answer
verified

Correct Answer
verified
Primers: 5'-CGGCTT-3' and 5'-G...
View Answer

Which of the following vectors can accommodate the largest DNA insert?

Correct Answer
verified

Exam 8: Recombinant Dna Technology and Molecular Cloning

What is suggested by the fact that type II restriction endonuclease recognition sequences are usually palindromic?

Correct AnswerverifiedShow Answer

To prepare a hybridization probe for screening a library, which labeling technique would be most desirable?

Correct AnswerverifiedShow Answer

Following the injection of a linear phage DNA into E. coli host cells, the phage genome circularizes by

Correct AnswerverifiedShow Answer

Explain the terms "random walk" and "coupling" in the context of the mode of action of restriction endonucleases.

Correct AnswerAnswered by ExamLex AIRandom walk refers to the movement of a ...View AnswerShow Answer

You want to join together two pieces of DNA that have been cut with SmaI. Outline a strategy you could use to increase the efficiency of blunt end ligation.

Correct AnswerAnswered by ExamLex AIOne strategy to increase the efficiency ...View AnswerShow Answer

Describe the principle of ion-exchange chromatography. Describe the behavior of a positively charged protein and a negatively charged protein in a column containing positively charged resin during sample loading and elution.

Correct AnswerAnswered by ExamLex AIIon-exchange chromatography is a techniq...View AnswerShow Answer

To read an old-fashioned Sanger sequencing gel in the 5' to 3' direction, on must read the gel

Correct AnswerverifiedShow Answer

You want to make a genomic library with DNA fragments averaging about 85 kb in length. Which vectors discussed would be appropriate to use? Which would be inappropriate? Explain your answer.

Correct AnswerAnswered by ExamLex AITo create a genomic library with DNA fra...View AnswerShow Answer

Explain why DNA polymerase from a thermophilic bacterium is used in PCR instead of DNA polymerase I from E. coli.

Correct AnswerAnswered by ExamLex AIDNA polymerase from a thermophilic bacte...View AnswerShow Answer

Which statement is not true about DNA binding and cleavage by type II restriction endonucleases?

Correct AnswerverifiedShow Answer

You want to clone a 1.5 kb cDNA. Which vectors discussed would be appropriate to use? Which would be inappropriate? Explain your answer.

Correct AnswerAnswered by ExamLex AIThe appropriate vectors to use for cloni...View AnswerShow Answer

What aspect of a common recombinant DNA technology tool makes direct use of knowledge acquired from studies of retrovirus replication?

Correct AnswerverifiedShow Answer

How can an amino acid sequence be used to design a gene-specific hybridization probe?

Correct AnswerverifiedShow Answer

What does it mean when some bands on an autoradiograph are darker than others?

Correct AnswerverifiedShow Answer

The appropriate hybridization temperature during library screening is calculated according to the G + C content and the percent homology of the probe to target. The more mismatches there are:

Correct AnswerverifiedShow Answer

In a single PCR cycle consisting of 30 seconds at 95C, 1 min at 55C, and 1 min at 72C, what is happening in the step run at 55C?

Correct AnswerverifiedShow Answer

Which step in Southern blotting allows an investigator to identify a particular DNA fragment that is present within a complex mixture of fragments?

Correct AnswerverifiedShow Answer

What is the difference between a restriction endonuclease recognition site and a RFLP?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedPrimers: 5'-CGGCTT-3' and 5'-G...View AnswerShow Answer

Which of the following vectors can accommodate the largest DNA insert?

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
Random walk refers to the movement of a ...
View Answer

Correct Answer
Answered by ExamLex AI
One strategy to increase the efficiency ...
View Answer

Correct Answer
Answered by ExamLex AI
Ion-exchange chromatography is a techniq...
View Answer

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
To create a genomic library with DNA fra...
View Answer

Correct Answer
Answered by ExamLex AI
DNA polymerase from a thermophilic bacte...
View Answer

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
The appropriate vectors to use for cloni...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
Primers: 5'-CGGCTT-3' and 5'-G...
View Answer

Correct Answer
verified