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Soil bacteria are incredibly resistant to multiple antibiotics,presumably as a response to competition from bacteria that produce antibiotics. What are three ways that these antibiotic resistant genes could move from soil bacteria species to other species of bacteria? Briefly describe each gene transfer process.Name one or more factors that would affect the likelihood of this transfer occurring.

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The three ways that genes can be passed ...

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This illustration represents: This illustration represents:   A) conjugation or transformation B) transduction or mutation C) genetic fusion D) protoplast fusion or gene amplification


A) conjugation or transformation
B) transduction or mutation
C) genetic fusion
D) protoplast fusion or gene amplification

E) C) and D)
F) None of the above

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In an experiment such as Griffith's pneumococcal experiment which of the following,when injected into a mouse,would result in its death?


A) Heat-killed, smooth pneumoccocci
B) Live, rough pneumococci
C) A mixture of heat killed smooth pneumococci and live rough pneumococci
D) A mixture of heat killed rough pneumococci and live rough pneumococci

E) A) and B)
F) All of the above

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The process of conjugation in bacteria requires that:


A) two types of viruses be present
B) the bacteria be deprived of nutrients
C) there be naked DNA in the environment
D) there be contact between donor and recipient cells

E) A) and B)
F) A) and D)

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A ________ is a genetic sequence able to move from one location in a chromosome to another.


A) plasmid
B) F factor
C) transposon
D) R factor

E) A) and B)
F) C) and D)

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Recombinant bacteria have been used to produce _____.


A) human growth hormone
B) vaccines
C) insulin
D) all of the above

E) A) and D)
F) All of the above

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During the process of lysogeny:


A) a phage integrates into the chromosome of the bacterium
B) a bacterium takes up DNA from the media
C) new phage particles are assembled in the host bacterium
D) a bacterium is split open to release new phage particles

E) A) and B)
F) C) and D)

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When genes are passed on from parent to offspring,which of the following events has taken place?


A) horizontal gene transfer
B) transduction
C) vertical gene transfer
D) retrograde gene transfer

E) C) and D)
F) B) and D)

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When cells conjugate for longer periods of time,which of the following will occur?


A) Fewer genes will be transferred.
B) More genes will be transferred.
C) The same number of genes will be transferred.
D) It is impossible to tell. Conjugation time and number of genes transferred are not related.

E) All of the above
F) B) and D)

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Bacterial conjugation is an example of _____.


A) transduction
B) vertical gene transfer
C) horizontal gene transfer
D) bacterial transformation

E) A) and B)
F) B) and D)

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In Griffith's transformation experiments,which of the following did not take place?


A) Live, capsulated pneumococci killed mice
B) Live unencapsulated bacteria were able to retrieve the DNA from dead encapsulated bacteria and become virulent
C) DNA was transferred from capsulated to unencapsulated bacteria using a bacteriophage
D) Dead, capsulated pneumococci had no effect on mice

E) B) and C)
F) B) and D)

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Plasmids can do which of the following?


A) Direct synthesis of conjugation pili
B) Provide resistance to certain antibiotics
C) Induce the formation of tumors in plants
D) All of the above are functions of various plasmids

E) C) and D)
F) B) and D)

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Hybridomas:


A) are responsible for tumor formation in plants
B) are resistant to monoclonal antibodies
C) divide and grow for only a short period of time
D) are the fusion of antibody producing plasma cell and a cancer cell

E) None of the above
F) B) and D)

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In specialized transduction the bacterial genes transduced tend to be:


A) those genes in greatest use by the bacteria
B) those genes that are currently not being transcribed
C) those genes that are located close to the site of the prophage insertion
D) those genes that are small enough to fit in the viral head

E) A) and B)
F) All of the above

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Gene amplification:


A) allows transposition of genes from one location on a chromosome to another
B) occurs when recombinant plasmids are induced to reproduce within cells at a rapid rate
C) allows material from one strain to recombine with that from another strain
D) complicate our ability to increase antibiotic production

E) B) and C)
F) B) and D)

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The genetic information needed for a cell to participate in conjugation resides in the DNA of a cell's _____.


A) bacterial chromosome
B) F plasmid
C) F pilus
D) mitochondria

E) None of the above
F) All of the above

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Natural selection:


A) acts on alleles already present in the population
B) explains how antibiotic resistance can be maintained in a population
C) doesn't consider competition between strains
D) two of the above

E) None of the above
F) B) and C)

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In a culture containing both F⁻ and F⁺ cells,which of the following will occur if we wait long enough?


A) No conjugation will occur
B) The cells will all become genetically identical
C) All F⁻ will become F⁺ cells
D) All F⁺ cells will become F⁻ cells

E) A) and D)
F) B) and C)

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Restriction enzymes come from _____.


A) fungal cells
B) viruses
C) bacterial cells
D) animal cells

E) A) and D)
F) A) and B)

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Hybridomas are used to produce large quantities of _____.


A) restriction enzymes
B) monoclonal antibodies
C) antibiotics
D) natural insecticides

E) All of the above
F) B) and D)

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