Question 1

The gold standard for the diagnosis of AML is based on what information?

Accepted Answer

A)  Immunophenotyping and morphology
B)  Morphology and cytogenetic studies
C)  Immunophenotyping and cytogenetic studies
D)  Cytochemical staining and immunophenotypingE) B) and D)
F) C) and D)

Question 2

The fluorescent dye employed by hematology analyzers to count reticulocytes binds to:

Accepted Answer

A)  DNA
B)  Proteins
C)  RNA
D)  Nucleic acidsE) B) and D)
F) None of the above

Question 3

Which two CD markers are helpful in differentially identifying CLL from hairy cell leukemia?

Accepted Answer

A)  CD19 and CD5
B)  CD5 and CD11c
C)  CD5 and CD25
D)  sIg and CD25E) B) and D)
F) None of the above

Question 4

What is the purpose of CD34 count?

Accepted Answer

To quantify stem cel...

Question 5

Which of the following is considered a drawback of immunophenotyping a suspected acute leukemia?

Accepted Answer

A)  AML NOC
B)  Lineage heterogeneity
C)  Lack of sensitivity
D)  Lack of specificityE) B) and C)
F) None of the above

Question 6

The EWGCCA recommends performing which of the following with CD34 enumeration?

Accepted Answer

A)  Dual staining with CD45
B)  DNA index
C)  IRF
D)  Exclude cells with low levels of CD 45 expressionE) A) and B)
F) None of the above

Question 7

Explain the advantages of using flow cytometry to count reticulocytes.

Accepted Answer

Quantification of reticulocytes using ma...

Question 8

An increased IRF is not seen in which of the following?

Accepted Answer

A)  Erythropoietin therapy
B)  Iron therapy in iron deficient patients
C)  Bone marrow recovery post-transplant
D)  Aplastic anemiaE) B) and C)
F) None of the above

Question 9

Which of the following is used to determine the relationship between the content of tumor cells and normal dividing cells?

Accepted Answer

A)  The absorbance concentration of the fluorochrome
B)  The DNA index
C)  The S phase fraction
D)  The G₂/M phase fractionE) A) and C)
F) A) and B)

Question 10

Immunophenotyping is essential for diagnosing ALL,separating T- and B- lineage ALL,and identifying subtypes of B lineage ALL.What markers can blasts express in precursor B cell ALL?

Accepted Answer

A)  CD10 and TdT
B)  CD1 and CD2
C)  CD4 and CD8
D)  CD41 and CD61E) C) and D)
F) A) and B)

Question 11

The following are accurate statements regarding fluorochromes except.

Accepted Answer

A)  They excite at one wavelength and emit light at a different wavelength.
B)  They can bind to DNA or RNA.
C)  They can be used to label detection antibodies.
D)  They contribute to the scattered light caused by a cell when it travels through the flowcellE) None of the above
F) All of the above

Question 12

For what reason is flow cytometry used in cell quantitation?

Accepted Answer

Flow cytometry can be used in ...

Question 13

Of the following,which is not an appropriate specimen for flow cytometry?

Accepted Answer

A)  Blood
B)  Bone marrow
C)  Lymphoid tissue
D)  PlasmaE) A) and B)
F) None of the above

Question 14

The flow cytometer contains a flow chamber that has two columns of fluid-sample and sheath-maintained at different pressure.Which of the following statements about the flow chamber is correct?

Accepted Answer

A)  Cells in the outer column become dispersed.
B)  The sample fluid has turbulent flow.
C)  Cells are aligned in single file in front of the laser.
D)  The two columns of fluid differ in color.E) A) and C)
F) A) and B)

Exam 40: Flow Cytometry

For what reason is flow cytometry used in cell quantitation?

Correct Answer
verified
Flow cytometry can be used in ...
View Answer

Which of the following is used to determine the relationship between the content of tumor cells and normal dividing cells?

Correct Answer
verified

The fluorescent dye employed by hematology analyzers to count reticulocytes binds to:

Correct Answer
verified

The gold standard for the diagnosis of AML is based on what information?

Correct Answer
verified

Explain the advantages of using flow cytometry to count reticulocytes.

Correct Answer
verified
Quantification of reticulocytes using ma...
View Answer

Which of the following is considered a drawback of immunophenotyping a suspected acute leukemia?

Correct Answer
verified

An increased IRF is not seen in which of the following?

Correct Answer
verified

Which two CD markers are helpful in differentially identifying CLL from hairy cell leukemia?

Correct Answer
verified

The flow cytometer contains a flow chamber that has two columns of fluid-sample and sheath-maintained at different pressure.Which of the following statements about the flow chamber is correct?

Correct Answer
verified

Of the following,which is not an appropriate specimen for flow cytometry?

Correct Answer
verified

The following are accurate statements regarding fluorochromes except.

Correct Answer
verified

Immunophenotyping is essential for diagnosing ALL,separating T- and B- lineage ALL,and identifying subtypes of B lineage ALL.What markers can blasts express in precursor B cell ALL?

Correct Answer
verified

What is the purpose of CD34 count?

Correct Answer
verified
To quantify stem cel...
View Answer

The EWGCCA recommends performing which of the following with CD34 enumeration?

Correct Answer
verified

Exam 40: Flow Cytometry

For what reason is flow cytometry used in cell quantitation?

Correct AnswerverifiedFlow cytometry can be used in ...View AnswerShow Answer

Which of the following is used to determine the relationship between the content of tumor cells and normal dividing cells?

Correct AnswerverifiedShow Answer

The fluorescent dye employed by hematology analyzers to count reticulocytes binds to:

Correct AnswerverifiedShow Answer

The gold standard for the diagnosis of AML is based on what information?

Correct AnswerverifiedShow Answer

Explain the advantages of using flow cytometry to count reticulocytes.

Correct AnswerverifiedQuantification of reticulocytes using ma...View AnswerShow Answer

Which of the following is considered a drawback of immunophenotyping a suspected acute leukemia?

Correct AnswerverifiedShow Answer

An increased IRF is not seen in which of the following?

Correct AnswerverifiedShow Answer

Which two CD markers are helpful in differentially identifying CLL from hairy cell leukemia?

Correct AnswerverifiedShow Answer

The flow cytometer contains a flow chamber that has two columns of fluid-sample and sheath-maintained at different pressure.Which of the following statements about the flow chamber is correct?

Correct AnswerverifiedShow Answer

Of the following,which is not an appropriate specimen for flow cytometry?

Correct AnswerverifiedShow Answer

The following are accurate statements regarding fluorochromes except.

Correct AnswerverifiedShow Answer

Immunophenotyping is essential for diagnosing ALL,separating T- and B- lineage ALL,and identifying subtypes of B lineage ALL.What markers can blasts express in precursor B cell ALL?

Correct AnswerverifiedShow Answer

What is the purpose of CD34 count?

Correct AnswerverifiedTo quantify stem cel...View AnswerShow Answer

The EWGCCA recommends performing which of the following with CD34 enumeration?

Correct AnswerverifiedShow Answer

Correct Answer
verified
Flow cytometry can be used in ...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
Quantification of reticulocytes using ma...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
To quantify stem cel...
View Answer

Correct Answer
verified