Question 1

Name at least two pitfalls in immunophenotyping mature lymphoid malignancies, and explain how you would correct this.

Accepted Answer

Some malignant B- cell lymphoproliferati...

Question 2

Choose the correct statement regarding the IRF from the choices below:

Accepted Answer

A)  The IRF is directly proportional to the degree of bone marrow turnover.
B)  The IRF is indirectly proportional to the average age of the reticulocytes.
C)  The IRF cannot be used to monitor erythropoietin therapy.
D)  The IRF cannot be used to monitor bone marrow recovery after transplant.E) B) and C)
F) A) and C)

Question 3

The fluorescent dye employed by hematology analyzers to count reticulocytes binds to:

Accepted Answer

A)  DNA.
B)  Proteins.
C)  RNA.
D)  Nucleic acids.E) C) and D)
F) A) and D)

Question 4

In diagnosing PNH, which of the following assays is most sensitive to the detection of the disorder?

Accepted Answer

A)  GPI anchor phenotype
B)  Ham test
C)  Sucrose lysis test
D)  Osmotic fragility testE) B) and C)
F) A) and B) A

Question 5

It is recommended by the EWGCCA to perform which of the following alongside CD34 enumeration?

Accepted Answer

A)  Dual staining with CD45
B)  DNA index
C)  IRF
D)  All of the aboveE) C) and D)
F) B) and D)

Question 6

Which of the following is considered a drawback of immunophenotyping a suspected acute leukemia?

Accepted Answer

A)  AML NOC
B)  Lineage heterogeneity
C)  Lack of sensitivity
D)  All of the aboveE) B) and C)
F) All of the above

Question 7

Neoplasms are made up of a population of identical cells, or a clone of cells.A clone, when identified, can lead to discovery of a lymphoid malignancy or other lymphoproliferative disorder.In what ways can clonality be detected?

Accepted Answer

A)  Uniform expression of one immunoglobulin light chain
B)  Mixture of cells with either kappa or lambda light chains
C)  Surface immunoglobulin detection
D)  Bright intensity of all monoclonal markersE) C) and D)
F) A) and B)

Question 8

Explain the advantages of using flow cytometry to count reticulocytes.

Accepted Answer

Quantification of reticulocytes using ma...

Question 9

Cells can be separated during data analysis by gating.This process of isolating cells can be based on what properties?

Accepted Answer

A)  Cell size and granularity
B)  Cell density
C)  Shape of the nucleus
D)  Color of the cytoplasmE) A) and D)
F) A) and C)

Question 10

DNA content of a patient's tumor cells is reported as 2.0, and the DNA content of diploid cells is 1.0.What is the DNA index?

Accepted Answer

A)  2.0
B)  3.0
C)  1.0
D)  0.5E) A) and B)
F) A) and C)

Question 11

A single group of cells possessing identical aberrant phenotypes is defined as:

Accepted Answer

A)  Monoclonal.
B)  Clonality.
C)  Tumorigenic.
D)  Contour gating.E) B) and C)
F) None of the above

Question 12

Which two CD markers would be helpful in differentially identifying CLL from hairy cell leukemia?

Accepted Answer

A)  CD19 and CD5
B)  CD5 and CD11c
C)  CD5 and CD25
D)  sIg and CD25E) None of the above
F) A) and D)

Question 13

The flow cytometer contains a flow chamber.This chamber has two columns of fluid maintained at different pressure, sample and sheath fluid.Which of the following statements about the flow chamber is correct?

Accepted Answer

A)  Cells in the outer column become dispersed.
B)  The sample fluid has turbulent flow.
C)  Cells are aligned in single file in front of the laser.
D)  The two columns of fluid differ in color.E) A) and B)
F) None of the above

Question 14

What is the purpose of CD34 count?

Accepted Answer

To quantify stem cel...

Question 15

What is one advantage to using monoclonal antibodies, rather than polyclonal antibodies, in immunophenotyping?

Accepted Answer

A)  They are commercially available.
B)  The antigen can be recognized even if some parts of it are abnormal.
C)  They have high purity and reproducibility.
D)  They can be directly attached to fluorochromes.E) None of the above
F) All of the above

Question 16

Which analyte is the best indicator of the overall picture of the immune system in an HIV-positive patient?

Accepted Answer

A)  Absolute CD4 count
B)  Relative lymphocyte count
C)  WBC count
D)  All of the aboveE) All of the above
F) A) and B)

Question 17

How can flow cytometry be used in cell quantitation?

Accepted Answer

Flow cytometry can be used in ...

Question 18

An unknown cell resembling a hematogone has CD19 and CD20 antigens.What is the lineage of the unknown cell?

Accepted Answer

A)  Myeloid
B)  Monocytic
C)  Lymphoid
D)  ErythroidE) A) and C)
F) C) and D) C

Question 19

CD34 enumeration is useful in bone marrow and peripheral blood stem cell transplantation.The EWGCCA recommendations for this enumeration include proper technique and interpretation of the flow cytometry analysis.Which of the following standards were recommended for proper acquisition of events?

Accepted Answer

A)  Include platelets and unlysed red blood cells and debris
B)  Include only CD34 dim staining populations
C)  Use bright fluorochrome conjugates of class II or III monoclonal antibodies that detect all glycoforms of CD34
D)  Acquire only 10 CD34 positive cellsE) B) and C)
F) A) and D) C

Question 20

Which of the following is/are appropriate specimen(s)  for flow cytometry?

Accepted Answer

A)  Blood
B)  Bone marrow
C)  Lymphoid tissue
D)  All of the aboveE) B) and C)
F) None of the above

Exam 36: Flow Cytometry

Name at least two pitfalls in immunophenotyping mature lymphoid malignancies, and explain how you would correct this.

Correct AnswerverifiedSome malignant B- cell lymphoproliferati...View AnswerShow Answer

Choose the correct statement regarding the IRF from the choices below:

Correct AnswerverifiedShow Answer

The fluorescent dye employed by hematology analyzers to count reticulocytes binds to:

Correct AnswerverifiedShow Answer

In diagnosing PNH, which of the following assays is most sensitive to the detection of the disorder?

Correct AnswerverifiedA

It is recommended by the EWGCCA to perform which of the following alongside CD34 enumeration?

Correct AnswerverifiedShow Answer

Which of the following is considered a drawback of immunophenotyping a suspected acute leukemia?

Correct AnswerverifiedShow Answer

Neoplasms are made up of a population of identical cells, or a clone of cells.A clone, when identified, can lead to discovery of a lymphoid malignancy or other lymphoproliferative disorder.In what ways can clonality be detected?

Correct AnswerverifiedShow Answer

Explain the advantages of using flow cytometry to count reticulocytes.

Correct AnswerverifiedQuantification of reticulocytes using ma...View AnswerShow Answer

Cells can be separated during data analysis by gating.This process of isolating cells can be based on what properties?

Correct AnswerverifiedShow Answer

DNA content of a patient's tumor cells is reported as 2.0, and the DNA content of diploid cells is 1.0.What is the DNA index?

Correct AnswerverifiedShow Answer

A single group of cells possessing identical aberrant phenotypes is defined as:

Correct AnswerverifiedShow Answer

Which two CD markers would be helpful in differentially identifying CLL from hairy cell leukemia?

Correct AnswerverifiedShow Answer

The flow cytometer contains a flow chamber.This chamber has two columns of fluid maintained at different pressure, sample and sheath fluid.Which of the following statements about the flow chamber is correct?

Correct AnswerverifiedShow Answer

What is the purpose of CD34 count?

Correct AnswerverifiedTo quantify stem cel...View AnswerShow Answer

What is one advantage to using monoclonal antibodies, rather than polyclonal antibodies, in immunophenotyping?

Correct AnswerverifiedShow Answer

Which analyte is the best indicator of the overall picture of the immune system in an HIV-positive patient?

Correct AnswerverifiedShow Answer

How can flow cytometry be used in cell quantitation?

Correct AnswerverifiedFlow cytometry can be used in ...View AnswerShow Answer

An unknown cell resembling a hematogone has CD19 and CD20 antigens.What is the lineage of the unknown cell?

Correct AnswerverifiedC

CD34 enumeration is useful in bone marrow and peripheral blood stem cell transplantation.The EWGCCA recommendations for this enumeration include proper technique and interpretation of the flow cytometry analysis.Which of the following standards were recommended for proper acquisition of events?

Correct AnswerverifiedC

Which of the following is/are appropriate specimen(s) for flow cytometry?

Correct AnswerverifiedShow Answer

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Some malignant B- cell lymphoproliferati...
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Quantification of reticulocytes using ma...
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To quantify stem cel...
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Flow cytometry can be used in ...
View Answer

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C

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C

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