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Exam 18: Practical Applications of Immunology

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Question 1

True/False

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A positive complement-fixation test is indicated by the lysis of the sheep red blood cells added in the indicator phase of the test.

A) True
B) False

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Question 2

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A reaction between antibody and particulate antigen is called a(n)

A) agglutination reaction.
B) complement fixation.
C) immunofluorescence.
D) neutralization reaction.
E) precipitation reaction.

F) A) and E)
G) B) and E)

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Question 3

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An injection of "naked" DNA into muscle cells to induce an immune response against the proteins encoded by the DNA is an example of a subunit vaccine.

What type of vaccine is the live,weakened measles virus?

Figure 18.1 -Figure 18.1 is an illustration of a(n)

Table 18.1 Antibody Titer $\begin{array} { | l | r | r | r | r | } \hline & \text { Day 1 } & { \text { Day 7 } } & \text { Day 14 } & \text { Day 21 } \\\hline \text { Patient A } & 0 & 0 & 256 & 512 \\\hline \text { Patient B } & 128 & 256 & 512 & 1024 \\\hline \text { Patient C } & 0 & 0 & 0 & 0 \\\hline \text { Patient D } & 128 & 128 & 128 & 128 \\\hline\end{array}$ -In Table 18.1,who showed seroconversion during these observations?

When producing monoclonal antibodies,the use of ________ is critical after fusion to specifically allow only hybrid cells to grow.

Western blotting uses DNA probes to detect specific sequences in a mixture of proteins.

Which of the following is a pregnancy test used to find the fetal hormone HCG in a woman's urine using anti-HCG and latex spheres?

If the table continued on to another week of testing,what is the one outcome below that you might NOT expect to see for Patient B at Day 28?

In an agglutination test,eight serial dilutions to determine antibody titer were set up.Tube #1 contained a 1:2 dilution;tube #2,a 1:4,etc.If tube #6 is the last tube showing agglutination,what is the antibody titer?

A patient's serum,Mycobacterium,guinea pig complement,sheep red blood cells,and anti-sheep red blood cell antibodies are mixed in a test tube.What happens if the patient has antibodies to Mycobacterium?

Table 18.1 Antibody Titer $\begin{array} { | l | r | r | r | r | } \hline & \text { Day 1 } & { \text { Day 7 } } & \text { Day 14 } & \text { Day 21 } \\\hline \text { Patient A } & 0 & 0 & 256 & 512 \\\hline \text { Patient B } & 128 & 256 & 512 & 1024 \\\hline \text { Patient C } & 0 & 0 & 0 & 0 \\\hline \text { Patient D } & 128 & 128 & 128 & 128 \\\hline\end{array}$ -In Table 18.1,who probably has the disease?

Which of the following is a test to determine the presence of soluble antigens in a patient's saliva?

Purified protein from Bordetella pertussis is used in a(n)

Vaccines are preparations of organisms or fractions of organisms that are used to induce protective immune responses.

Agglutination tests use particulate antigens while precipitation tests use soluble antigens.

The home pregnancy test kit is an example of a direct ELISA.

Blood typing tests are examples of hemagglutination reactions.

Which of the following is NOT an advantage of live attenuated vaccine agents?