Question 1

In bacteria, initiator tRNA carries formyl methionine.

Accepted Answer

A) True 
 B)False

Question 2

The enzyme that helps pack DNA into the cell by coiling the DNA into a tight bundle is _______.

Accepted Answer

A)  DNA ligase
B)  DNA polymerase
C)  DNA helicase
D)  DNA gyrase
E)  primaseF) B) and E)
G) B) and C)

Question 3

DNA polymerase can catalyze a dehydration synthesis reaction only at the 3'carbon of the daughter strand of DNA. This means that

Accepted Answer

A)  	the antiparallel nature of the parent molecules determine that the leading strand must be grown in fragments away from the fork and the lagging strand is synthesized continuously toward the fork.
B)  	the leading and lagging strands extend in the 3'- 5'direction toward the replication fork as the nucleotide bases are exposed.
C)  	Okazaki fragments extend toward the replication fork as the parent strands are unzipped by helicase.
D)  	since the strands are antiparallel, one strand will grow toward the fork as it opens, and the other strand will grow in fragments away from the fork.E) B) and D)
F) None of the above

Question 4

DNA polymerase can only add new nucleotides to the 3'DNA end of the template.

Accepted Answer

A) True 
 B)False

Question 5

Hfr transfer involves all of the following except

Accepted Answer

A)  gene integration into the bacterial chromosome.
B)  independent plasmid transfer.
C)  F factor is part of the F+ donor chromosome.
D)  high frequency transfer.
E)  a pilus connection between F+ and F- cells.F) A) and E)
G) A) and B)

Question 6

If the wild type DNA sequence reads THE CAT ATE THE BIG RAT, what type of mutation would change the sequence to THE CAT ATE (stop) ?

Accepted Answer

A)  Missense
B)  Nonsense
C)  Insertion
D)  Deletion
E)  SilentF) C) and E)
G) B) and C)

Question 7

A screening system called the _______ test is used for detecting chemicals with carcinogenic potential.

Accepted Answer

A)  Koch
B)  Ames
C)  mutation
D)  cancer
E)  IowaF) A) and D)
G) None of the above

Question 8

A sequence of bases within a gene that does not code for protein is called a/n _______.

Accepted Answer

A)  promoter
B)  operator
C)  operon
D)  exon
E)  intronF) B) and E)
G) C) and D)

Question 9

The DNA of an organism is made up of subunits called _______.

Accepted Answer

A)  histones
B)  amino acids
C)  nucleotides
D)  mRNA
E)  polymerasesF) A) and B)
G) C) and E)

Question 10

Which is incorrect about purines?

Accepted Answer

A)  They are only found in DNA, not in RNA.
B)  They are nitrogenous bases.
C)  They are always paired with a specific pyrimidine.
D)  They include adenine and guanine.
E)  They are found within nucleotides.F) A) and E)
G) A) and D)

Question 11

The following pertain to ribosomes during protein synthesis except

Accepted Answer

A)  they contain codons within their rRNA molecules.
B)  they participate only in translation.
C)  they bind to the 5'end of mRNA by their small subunit.
D)  they use their large subunit to supply enzymes for peptide bonding.
E)  they shift towards the 3'direction along the mRNA strand from one codon to the next.F) A) and B)
G) C) and D)

Question 12

In order for protein synthesis to begin, which of the following statements reflects the correct sequence of events?

Accepted Answer

A)  	The AUG codon on mRNA base pairs with the UAC anticodon on tRNA allowing the small and large ribosomal subunits to join the complex.
B)  	Translation begins when the ribosome translocates from the A site to the P site on the mRNA
C)   The 5'end of the mRNA binds to the small ribosomal subunit, and the large subunit joins, creating the P and A sites where reactions between codons on the mRNA and anticodons on tRNA are stabilized.
D)   The 3'end of the mRNA binds to the small ribosomal subunit and the AUG anticodon on the tRNA base pairs with the UAC codon on the mRNA, allowing the large ribosomal subunit to attach.E) A) and D)
F) None of the above

Question 13

A permanent, inheritable change in the genetic information is called ________.

Accepted Answer

A)  translation
B)  transcription
C)  a mutation
D)  an alteration
E)  regenerationF) C) and D)
G) None of the above

Question 14

Induced mutations are initiated by errors in DNA replication.

Accepted Answer

A) True 
 B)False

Question 15

Repressible operons require that ______ binds to the repressor protein before it can bind to the operator.

Accepted Answer

A)  the product
B)  a cofactor
C)  a coenzyme
D)  the substrate
E)  the reactantF) None of the above
G) B) and E)

Question 16

A bacterial cell has replicated its plasmid, and when this cell divides into 2 daughter cells, each cell receives one copy of the plasmid.This is an example of horizontal gene transfer.

Accepted Answer

A) True 
 B)False

Question 17

The transfer of DNA fragments from a dead cell to a live, competent recipient cell that results in a changed recipient cell is termed ________.

Accepted Answer

A)  transduction
B)  conjugation
C)  transformation
D)  transmission
E)  mitosisF) B) and D)
G) C) and E)

Question 18

The field of genomics refers to

Accepted Answer

A)  the study of an organism's complete genome, including plasmid, mitochondrial, and chloroplast DNA.
B)  the study of chromosomal DNA.
C)  the study of an organism's plasmids.
D)  the study of cellular, but not viral, genomes.E) B) and D)
F) A) and D)

Question 19

Of the following list, which antibiotics affect protein synthesis?

Accepted Answer

A)  Penicillin
B)  Tetracycline
C)  Chloramphenicol
D)  Ciprofloxacin
E)  Both tetracycline and chloramphenicol are correct.F) A) and E)
G) A) and B)

Question 20

Structural genes code for

Accepted Answer

A)  ribosomal RNA molecules.
B)  transfer RNA molecules.
C)  cellular proteins.
D)  gene expression elements.E) All of the above
F) A) and B)

Exam 9: Microbial Genetics

Structural genes code for

Correct AnswerverifiedShow Answer

The DNA of an organism is made up of subunits called _______.

Correct AnswerverifiedShow Answer

In order for protein synthesis to begin, which of the following statements reflects the correct sequence of events?

Correct AnswerverifiedShow Answer

The enzyme that helps pack DNA into the cell by coiling the DNA into a tight bundle is _______.

Correct AnswerverifiedShow Answer

A sequence of bases within a gene that does not code for protein is called a/n _______.

Correct AnswerverifiedShow Answer

The following pertain to ribosomes during protein synthesis except

Correct AnswerverifiedShow Answer

Of the following list, which antibiotics affect protein synthesis?

Correct AnswerverifiedShow Answer

The transfer of DNA fragments from a dead cell to a live, competent recipient cell that results in a changed recipient cell is termed ________.

Correct AnswerverifiedShow Answer

In bacteria, initiator tRNA carries formyl methionine.

Correct AnswerverifiedShow Answer

The field of genomics refers to

Correct AnswerverifiedShow Answer

Repressible operons require that ______ binds to the repressor protein before it can bind to the operator.

Correct AnswerverifiedShow Answer

DNA polymerase can catalyze a dehydration synthesis reaction only at the 3'carbon of the daughter strand of DNA. This means that

Correct AnswerverifiedShow Answer

A permanent, inheritable change in the genetic information is called ________.

Correct AnswerverifiedShow Answer

Hfr transfer involves all of the following except

Correct AnswerverifiedShow Answer

DNA polymerase can only add new nucleotides to the 3'DNA end of the template.

Correct AnswerverifiedShow Answer

A bacterial cell has replicated its plasmid, and when this cell divides into 2 daughter cells, each cell receives one copy of the plasmid.This is an example of horizontal gene transfer.

Correct AnswerverifiedShow Answer

Which is incorrect about purines?

Correct AnswerverifiedShow Answer

A screening system called the _______ test is used for detecting chemicals with carcinogenic potential.

Correct AnswerverifiedShow Answer

Induced mutations are initiated by errors in DNA replication.

Correct AnswerverifiedShow Answer

If the wild type DNA sequence reads THE CAT ATE THE BIG RAT, what type of mutation would change the sequence to THE CAT ATE (stop) ?

Correct AnswerverifiedShow Answer

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