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Exam 17: Recombinant DNA Technology

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Question 21

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The PCR method was developed by

A) Boyer.
B) Mullis.
C) Cohen.
D) Sanger.

E) B) and C)
F) C) and D)

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Question 22

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Which is most analogous to the role of GFP in recombinant DNA technology?

A) Using dyes to stain and detect bacteria under light microscopy from the sputum of a patient diagnosed with tuberculosis.
B) Comparing the RFLP patterns of DNA from various suspects to DNA isolated from the scene of a crime using gel electrophoresis.
C) Creating a metagenomic library representing archaea bacteria that thrive in the hot springs of Yellowstone National Park.
D) Monitoring the spread of multi-drug resistant pathogens on a hospital floor by comparing antibiotic susceptibility patterns.

E) B) and D)
F) A) and B)

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Question 23

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Plasmid DNA having one EcoRI recognition sequence is treated with EcoRI restriction endonuclease.Following gel electrophoresis,how many bands should be visible on the gel?

Which of the following bacterial hosts would should be used to avoid degradation of DNA that is introduced via a cloning vector?

Cosmids are plasmids that can be packaged into capsids of the bacteriophage lambda; therefore,they can be transmitted like phages,but they can exist and replicate in a cell like plasmids.

One of the major advantages to using plasmids as cloning vectors is that very high copy numbers can be achieved with many types of plasmid vectors.

During the investigation of a convenience store robbery,witnesses report that the perpetrator exited through a main entry door without wearing gloves.The police are holding a prime suspect in custody while samples from the door are collected and analyzed for a DNA match.Since the door was handled by many people before the robbery,how will the forensics department distinguish the suspect's DNA from the DNA of others using PCR?

The Southern blotting technique for transferring DNA from an agarose gel to a piece of nitrocellulose membrane is named for

Which cloning vector should be used to express a 500-kb DNA fragment in Saccharomyces cerevisiae?

Recombinant DNA technology does not rely on which of the following enzymes?

___________ is a bacterial plasmid vector.

Which is a true statement regarding the size of PCR products?

Which of the following types of cloning vector can carry the largest amount of foreign DNA?

A (n) __________ vector contains promoters that result in high-level transcription of the gene cloned within a multicloning site.

Genetic engineering methods have been used to produce vaccines.

As a newly hired molecular biologist for a company that produces genetically modified seeds,your first project is to ensure that a GM cotton plant carrying a bacterial insecticide gene is expressed in tissues of seedlings as well as mature plants.To better understand regulation of the gene,you decide to use GFP fusion to detect activity of the promoter and also use GFP fusion to determine the location of the protein in plants tissues at various stages of growth.

A molecular biologist is interested in purify a recombinant protein by His-tagging,but the protein and vector both lack histidine residues.In this case,the molecular biologist could use which of the following technique(s) to acheive purification?

Cosmids are so named because they can be used to express foreign genes in a variety of different hosts.

If all of the PCR products of a DNA sequence are 4000 bp,then the DNA sequence that was amplified must have been 8000 bp.

Antibiotics incorporated into the culture medium can