Question 1

Which of the following techniques exemplifies phenotypic rescue in a host cell?

Accepted Answer

A)  Introducing a genomic library from a cell that produces a particular gene product into a mutant host cell auxotroph that cannot synthesize the product.
B)  Introducing a genomic library from a mutant cell auxotroph for a particular gene product into a host cell that can synthesize that product constitutively.
C)  Removing genes that encode a particular gene product from a cell that expresses that product constitutively before creating a genomic library.
D)  Replacing a mutant gene with a functional gene in a DNA library from an auxotrophic mutant cell prior to introducing the library into a related host cell.E) A) and C)
F) B) and D)

Question 2

A microbial ecologist who is interested in studying how gene expression is influenced by different species of bacteria in a biofilm would most likely find a metagenomic library more useful than a genomic library.

Accepted Answer

A) True 
 B)False

Question 3

Which of the following components of PCR ensures amplification of the target DNA sequence only,despite the presence of many other DNA sequences?

Accepted Answer

A)  Primers
B)  Polymerases
C)  dNTPs
D)  High temperaturesE) None of the above
F) All of the above

Question 4

Which of the following is not true of cloning vectors?

Accepted Answer

A)  They usually contain multicloning sites or polylinkers.
B)  They contain at least two replication origins.
C)  They can be replicated within an appropriate host.
D)  All of these are true of cloning vectors.E) C) and D)
F) All of the above

Question 5

Plasmid cloning vector DNA is usually introduced into bacterial hosts by

Accepted Answer

A)  ligation.
B)  transformation.
C)  transduction.
D)  plasmolysis.E) A) and B)
F) B) and D)

Question 6

Restriction endonucleases in bacteria may have evolved in order to

Accepted Answer

A)  carry out natural genetic engineering.
B)  protect the bacteria from infection by viruses.
C)  use nucleic acids as a food (energy) source.
D)  All of the choices are correct.E) A) and B)
F) None of the above

Question 7

In _____________,cells are mixed with recombinant DNA and exposed to a brief pulse of high-voltage electricity to cause the membrane to become permeable and allow the uptake of DNA from its environment.

Accepted Answer

The answer of In _____________,cells are mixed with recombinant DNA...

Question 8

If all of the restriction fragments in a DNA digest were of very low molecular weight,then all of the fragments on the electrophoretic gel will be closest to the positive pole and furthest from the negative pole.

Accepted Answer

A) True 
 B)False

Question 9

Which of the following terms is most closely related to genetic complementation?

Accepted Answer

A)  Phenotypic rescue
B)  Naked DNA
C)  Reverse transcription
D)  SOS responseE) A) and B)
F) A) and C)

Question 10

Regardless of the exact approach taken to recombinant DNA technology,one of the keys to successful cloning is choosing the right vector.

Accepted Answer

A) True 
 B)False

Exam 17: Recombinant DNA Technology

Plasmid cloning vector DNA is usually introduced into bacterial hosts by

Correct Answer
verified

Which of the following techniques exemplifies phenotypic rescue in a host cell?

Correct Answer
verified

If all of the restriction fragments in a DNA digest were of very low molecular weight,then all of the fragments on the electrophoretic gel will be closest to the positive pole and furthest from the negative pole.

Correct Answer
verified

Which of the following is not true of cloning vectors?

Correct Answer
verified

Which of the following terms is most closely related to genetic complementation?

Correct Answer
verified

A microbial ecologist who is interested in studying how gene expression is influenced by different species of bacteria in a biofilm would most likely find a metagenomic library more useful than a genomic library.

Correct Answer
verified

Which of the following components of PCR ensures amplification of the target DNA sequence only,despite the presence of many other DNA sequences?

Correct Answer
verified

Restriction endonucleases in bacteria may have evolved in order to

Correct Answer
verified

In _____________,cells are mixed with recombinant DNA and exposed to a brief pulse of high-voltage electricity to cause the membrane to become permeable and allow the uptake of DNA from its environment.

Correct Answer
verified

Regardless of the exact approach taken to recombinant DNA technology,one of the keys to successful cloning is choosing the right vector.

Correct Answer
verified

Exam 17: Recombinant DNA Technology

Plasmid cloning vector DNA is usually introduced into bacterial hosts by

Correct AnswerverifiedShow Answer

Which of the following techniques exemplifies phenotypic rescue in a host cell?

Correct AnswerverifiedShow Answer

If all of the restriction fragments in a DNA digest were of very low molecular weight,then all of the fragments on the electrophoretic gel will be closest to the positive pole and furthest from the negative pole.

Correct AnswerverifiedShow Answer

Which of the following is not true of cloning vectors?

Correct AnswerverifiedShow Answer

Which of the following terms is most closely related to genetic complementation?

Correct AnswerverifiedShow Answer

A microbial ecologist who is interested in studying how gene expression is influenced by different species of bacteria in a biofilm would most likely find a metagenomic library more useful than a genomic library.

Correct AnswerverifiedShow Answer

Which of the following components of PCR ensures amplification of the target DNA sequence only,despite the presence of many other DNA sequences?

Correct AnswerverifiedShow Answer

Restriction endonucleases in bacteria may have evolved in order to

Correct AnswerverifiedShow Answer

In _____________,cells are mixed with recombinant DNA and exposed to a brief pulse of high-voltage electricity to cause the membrane to become permeable and allow the uptake of DNA from its environment.

Correct AnswerverifiedShow Answer

Regardless of the exact approach taken to recombinant DNA technology,one of the keys to successful cloning is choosing the right vector.

Correct AnswerverifiedShow Answer

Correct Answer
verified

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verified

Correct Answer
verified

Correct Answer
verified

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verified

Correct Answer
verified

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verified

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verified

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verified

Correct Answer
verified