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Quorum sensing in Gram-positive bacteria generally involves the use of ____________ as the external signaling molecules.

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The process of segregating the daughter chromosomes in Sulfolobus is more similar to the process in eukaryotic cells than in bacterial cells.

A) True
B) False

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Which of the following might be missed if viable counts are done on food and/or water specimens?


A) Endospores
B) Aerobic bacteria
C) Viable but nonculturable cells
D) Pathogenic bacteria

E) None of the above
F) B) and D)

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Agar is an excellent solidifying agent for microbiological media because


A) it is not degraded by most microorganisms.
B) solid agar remains solid until the temperature is raised to 90 \circ C,and liquid agar remains liquid if the temperature is lowered to 45 \circ C.
C) it is not degraded by most microorganisms and solid agar remains solid until the temperature is raised to 90 \circ C,and liquid agar remains liquid if the temperature is lowered to 45 \circ C are correct.
D) agar provides an excellent source of nutrition for a variety of different microorganisms.

E) A) and B)
F) B) and C)

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The process of forming a cross wall between two daughter cells is known as _______________.


A) replication
B) septation
C) sporulation
D) mitosis

E) A) and C)
F) B) and C)

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You would like to culture a bacterium that is growing in the lung of a human patient with pneumonia.Which media would be of most use in culturing this organism?


A) Transport media
B) Mannitol salt agar
C) MacConkey's agar
D) Complex medium supplemented with whole blood
E) None of the choices are correct.

F) B) and E)
G) A) and B)

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Organisms that grow well at 0 \circ C and have optimum growth temperatures of 15 \circ C or lower are called


A) psychrotrophs.
B) psychrophiles.
C) frigiphiles.
D) mesophiles.

E) C) and D)
F) A) and B)

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A microbiologist is trying to isolate a pathogen expected to be present in very small numbers from a stool specimen in which there are abundant normal microbiota.Which strategy would most likely accomplish this purpose?


A) Culture on enriched media
B) Enrichment culture followed by culture on selective agar
C) Culture on differential media
D) Culture in an anaerobe jar

E) A) and B)
F) A) and C)

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For surface cultivation of microorganisms,a sulfated polymer called agar can be extracted from __________ and added to liquid media in order to cause it to solidify.


A) bacteria
B) animal fat
C) algae
D) fungi

E) B) and D)
F) All of the above

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Mannitol salt agar (MSA) only allows the growth of halophiles (salt-loving microbes) ; nonhalophiles will not grow.Among the halophiles,mannitol fermenters will produce acid that turns the pH indicator yellow; mannitol nonfermenters leave the medium red.Onto MSA you inoculate a halophilic mannitol fermenter and a halophilic mannitol nonfermenter.In this case,the medium is acting as (a) __________ medium(s) .


A) selective
B) differential
C) selective and differential
D) enrichment

E) A) and D)
F) All of the above

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What is the result of successful communication between rhizobia and their plant hosts?


A) Rhizobia consume the plant tissue as an energy and carbon source,causing disease in the plant.
B) The plant consumes rhizobiaas an energy source and carbon,destroying the invading bacteria.
C) The rhizobia invade the plant root and begin to fix nitrogen with the assistance of the plant.
D) The plant supplies organic forms of nitrogen to the rhizobia.

E) All of the above
F) B) and D)

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An open system in which the growth rate is maintained by the removal and addition of media at such a rate as to maintain a constant cell density is called a


A) manostat.
B) chemostat.
C) turbidostat.
D) culturostat.

E) A) and C)
F) B) and C)

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Media containing some ingredients of unknown chemical composition are called __________ media.


A) undefined
B) complex
C) defined
D) synthetic

E) A) and D)
F) A) and C)

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A given medium can be either selective or differential but not both.

A) True
B) False

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Complex microbial communities that grow tightly adhered to surfaces are called __________.

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Most microorganisms live in eutrophic environments.

A) True
B) False

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The site at which replication of DNA starts in bacterial cells is known as the __________ of ___________.

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A growth medium that distinguishes among different groups of bacteria on the basis of their biological characteristics is called a __________ medium.


A) selective
B) differential
C) enrichment
D) transport

E) All of the above
F) C) and D)

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Microbial __________ is an increase in cell constituents that may or may not be accompanied by an increase in cell number.

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Media in which all components and their concentration are known are called __________ media.


A) transport
B) defined
C) selective
D) enrichment

E) All of the above
F) C) and D)

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