Question 1

The term 'recombinant organism' refers to

Accepted Answer

A)   an organism that has genes passed down from the parent cell to the daughter cells during binary fission.
B)   genetically engineered cells, but not those that have new genes as a result of horizontal gene transfer.
C)   an organism that expresses proteins coded for by genes that originated in a different organism.
D)   an organism that has recombined its genes.E) All of the above
F) A) and B)

Question 2

Recombinant organisms are those that have received new DNA in the form of a conjugated plasmid; genetically engineered cells are not considered recombinant.

Accepted Answer

A) True 
 B)False  False

Question 3

Split genes

Accepted Answer

A)   are common in bacteria and eukaryotes.
B)   only have exons initially transcribed to mRNA.
C)   have introns located only at the beginning and end of a coding region.
D)   are acted upon by spliceosomes to excise introns and then join exons.E) B) and D)
F) A) and D)

Question 4

Synthesis of an inducible enzyme requires

Accepted Answer

A)   repressor alone bound to operator.
B)   substrate bound to repressor.
C)   substrate bound to promoter.
D)   corepressor and repressor binding to operator.E) All of the above
F) B) and D)

Question 5

In bacteria,initiator tRNA carries formyl methionine.

Accepted Answer

A) True 
 B)False

Question 6

The process in which mutations are removed and the correct bases added is called ________.

Accepted Answer

A)   transduction
B)   excision repair
C)   frameshift
D)   back-mutation
E)   transformationF) C) and E)
G) A) and B) B

Question 7

Okazaki fragments are attached to the growing end of the lagging strand by _______.

Accepted Answer

A)   DNA ligase
B)   DNA polymerase
C)   DNA helicase
D)   DNA gyrase
E)   primaseF) All of the above
G) B) and E)

Question 8

During replication,each parent DNA strand serves as a _____ for synthesis of new DNA strands.

Accepted Answer

A)   copy point
B)   template
C)   comparison molecule
D)   scaffold
E)   reservoirF) A) and C)
G) C) and D)

Question 9

The site where the old DNA strands separate and new DNA strands will be synthesized is called the ________.

Accepted Answer

A)   primer
B)   Okazaki fragment
C)   template
D)   rolling circle
E)   replication forkF) C) and D)
G) B) and D)

Question 10

The operon segment composed of the gene that codes for a protein repressor is called the ________.

Accepted Answer

A)   operator
B)   structural locus
C)   regulator
D)   promoterE) B) and C)
F) None of the above

Question 11

Looking at your results of the Ames test using bacteriological agar medium plates lacking the amino acid histidine,you find that there are many colonies growing on the agar.How do you interpret this result?

Accepted Answer

A)   The bacterium has turned its operon genes on, producing proteins necessary for growth.
B)   The chemical being tested is mutagenic.
C)   The bacterium does not have any plasmids, so it cannot grow on the medium.
D)   The drug being tested has no inhibitory effect on the bacterium; therefore, it is not a good antibiotic to give for treatment of disease.E) All of the above
F) A) and D)

Question 12

Helicase ______.

Accepted Answer

A)   unwinds DNA
B)   supercoils DNA
C)   unwinds RNA
D)   winds RNAE) A) and B)
F) B) and C)

Question 13

The RNA molecules that carry amino acids to the ribosomes during protein synthesis are called ________.

Accepted Answer

A)   ribosomal RNA
B)   messenger RNA
C)   transfer RNA
D)   primer RNA
E)   ribozymesF) A) and B)
G) A) and C)

Question 14

If the wild type DNA sequence reads THE CAT ATE THE BIG RAT,what type of mutation would change the sequence to THE CAT ATA ETH EBI GRA T?

Accepted Answer

A)   Missense
B)   Nonsense
C)   Insertion
D)   Deletion
E)   SilentF) A) and B)
G) B) and D)

Question 15

Gene regulation can involve a protein repressor that blocks ______ from initiating transcription.

Accepted Answer

A)   DNA polymerase I
B)   DNA polymerase III
C)   RNA polymerase
D)   mRNA
E)   rRNAF) A) and C)
G) A) and E)

Question 16

Introns have no detectable functions.

Accepted Answer

A) True 
 B)False

Question 17

Hfr transfer involves all of the following except

Accepted Answer

A)   gene integration into the bacterial chromosome.
B)   independent plasmid transfer.
C)   F factor is part of the F+ donor chromosome.
D)   high frequency transfer.
E)   a pilus connection between F+ and F- cells.F) D) and E)
G) A) and C)

Question 18

RNA polymerase binds to the

Accepted Answer

A)   start codon.
B)   termination sequence.
C)   regulation sequence.
D)   promoter sequence.E) A) and C)
F) All of the above

Question 19

You have taken E.coli strain A which has its own plasmids,and mixed it with E.coli B strain without plasmids.E.coli B cells now have plasmids but,in addition,they also carry some genes from E.coli A strain.What has happened is

Accepted Answer

A)  the E.coli B cells have become mutants of the original culture.
B)  vertical transmission of DNA has occurred.
C)  an example of transformation.
D)  the cells of E.coli A were Hfr cells.E) A) and B)
F) All of the above D

Question 20

All mutations are not beneficial to the cell.

Accepted Answer

A) True 
 B)False

Exam 9: Microbial Genetics

The term 'recombinant organism' refers to

Correct AnswerverifiedShow Answer

Recombinant organisms are those that have received new DNA in the form of a conjugated plasmid; genetically engineered cells are not considered recombinant.

Correct AnswerverifiedFalse

Split genes

Correct AnswerverifiedShow Answer

Synthesis of an inducible enzyme requires

Correct AnswerverifiedShow Answer

In bacteria,initiator tRNA carries formyl methionine.

Correct AnswerverifiedShow Answer

The process in which mutations are removed and the correct bases added is called ________.

Correct AnswerverifiedB

Okazaki fragments are attached to the growing end of the lagging strand by _______.

Correct AnswerverifiedShow Answer

During replication,each parent DNA strand serves as a _____ for synthesis of new DNA strands.

Correct AnswerverifiedShow Answer

The site where the old DNA strands separate and new DNA strands will be synthesized is called the ________.

Correct AnswerverifiedShow Answer

The operon segment composed of the gene that codes for a protein repressor is called the ________.

Correct AnswerverifiedShow Answer

Looking at your results of the Ames test using bacteriological agar medium plates lacking the amino acid histidine,you find that there are many colonies growing on the agar.How do you interpret this result?

Correct AnswerverifiedShow Answer

Helicase ______.

Correct AnswerverifiedShow Answer

The RNA molecules that carry amino acids to the ribosomes during protein synthesis are called ________.

Correct AnswerverifiedShow Answer

If the wild type DNA sequence reads THE CAT ATE THE BIG RAT,what type of mutation would change the sequence to THE CAT ATA ETH EBI GRA T?

Correct AnswerverifiedShow Answer

Gene regulation can involve a protein repressor that blocks ______ from initiating transcription.

Correct AnswerverifiedShow Answer

Introns have no detectable functions.

Correct AnswerverifiedShow Answer

Hfr transfer involves all of the following except

Correct AnswerverifiedShow Answer

RNA polymerase binds to the

Correct AnswerverifiedShow Answer

You have taken E.coli strain A which has its own plasmids,and mixed it with E.coli B strain without plasmids.E.coli B cells now have plasmids but,in addition,they also carry some genes from E.coli A strain.What has happened is

Correct AnswerverifiedD

All mutations are not beneficial to the cell.

Correct AnswerverifiedShow Answer

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False

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B

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D

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