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When some bacteria use lactose as a source of energy, they produce propionic acid and carbon dioxide. What is the name for this process?


A) fermentation
B) cellular respiration
C) chemical synthesis
D) photosynthesis
E) carbon fixation

F) A) and B)
G) A) and E)

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Researchers have found a new type of bacteria, and they want to determine its phylum. What would be the most reliable method to do so?


A) conducting a Gram-stain test
B) performing an analysis of metabolic pathways
C) determining the ribosomal RNA sequence of the bacteria
D) analyzing the morphological characteristics of the bacteria

E) B) and D)
F) All of the above

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Broad-spectrum antibiotics inhibit the growth of most intestinal bacteria. Consequently, assuming that nothing is done to counter the reduction of intestinal bacteria, a hospital patient who is receiving broad-spectrum antibiotics is most likely to become ________.


A) unable to fix carbon dioxide
B) antibiotic-resistant
C) unable to fix nitrogen
D) unable to synthesize peptidoglycan
E) deficient in certain vitamins and nutrients

F) D) and E)
G) A) and C)

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A biologist trying to determine the mechanism of adenosine triphosphate (ATP) production in a newly discovered bacterium provides several different substrates on which the bacteria can feed. Which of the following results would indicate that the species uses fermentation to produce ATP?


A) The bacteria utilize glucose and produce carbon dioxide as a by-product of respiration.
B) After the bacteria break down their substrate, the biologist notices a rotten egg smell.
C) The biologist discovers that the bacteria are able to produce organic molecules by metabolizing methane.
D) After exposure to amino acids, the bacteria produce a smell like rotting flesh.

E) All of the above
F) A) and B)

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An organic solvent widely used in industrial processes, 1,1,1-trichloroethane (TCA) , is a major environmental pollutant affecting human health and damaging the ozone layer. Recently, researchers isolated the first bacteria able to degrade TCA. They found that the bacteria use hydrogen as an electron donor, TCA as an electron acceptor, and acetate as a carbon source. Based on the preceding information, deduce the method used to isolate these bacteria.


A) Gram staining
B) enrichment culture
C) direct sequencing
D) seeding

E) None of the above
F) All of the above

Correct Answer

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Plantlike photosynthesis that releases oxygen (O2) occurs in ________.


A) cyanobacteria
B) chlamydias
C) archaea
D) actinomycetes
E) chemoautotrophic bacteria

F) A) and B)
G) A) and C)

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A prokaryote that obtains carbon and energy by ingesting prey is a(n) ________.


A) autotroph
B) chemotroph
C) autotroph and phototroph
D) heterotroph and chemotroph
E) autotroph, phototroph, and chemotroph

F) B) and C)
G) A) and E)

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You might be interested to know how many different types of bacteria live on the shower curtain in your bathroom. What is the most efficient method for finding out?


A) producing a molecular phylogeny
B) enrichment culture
C) direct sequencing
D) Enrichment culture and direct sequencing are equally efficient methods.

E) All of the above
F) C) and D)

Correct Answer

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Microbiologists use the Gram stain to aid in the identification of bacteria. What is the major difference between Gram-positive and Gram-negative bacteria?


A) structure of nucleotides in the plasma membrane
B) presence or absence of muramic acid in the cell wall
C) presence or absence of peptidoglycan in the cell wall
D) presence or absence of outer plasma membrane

E) C) and D)
F) All of the above

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A bacterium was grown in two test tubes filled with media rich in glucose. One tube was sealed to produce anaerobic conditions, and the other was not. A third uninoculated, unsealed tube was used as a control. Glucose utilization by this bacterium causes acid production, which is indicated by a lightening of the media color. If the bacterium is able to produce ATP only by aerobic respiration, we expect lightening of media in ________.


A) the sealed tube only
B) the unsealed tube only
C) the sealed and unsealed tubes only
D) the control tube only
E) all three tubes

F) B) and C)
G) B) and D)

Correct Answer

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The purple nonsulfur bacterium Rhodospirillum grows best as a photoheterotroph. What are the most favorable sources of energy and carbon for this bacterium?


A) methane and carbon dioxide
B) fructose and light
C) light and carbon dioxide
D) glucose

E) B) and C)
F) A) and D)

Correct Answer

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Some researchers have begun attempting to clean up oil spills by adding nonindigenous microbial hydrocarbon degraders to the spill, in the hope that these bacteria will neutralize the dangerous chemicals in the spill. This is an example of ________.


A) bioremediation via fertilization
B) seeding
C) production of a new antibiotic
D) use of extremophiles for cleanup of organic solvents

E) None of the above
F) A) and B)

Correct Answer

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Multicellularity and large body size of eukaryotic organisms require high metabolic rates and efficient ATP production by aerobic respiration. How did bacteria change Earth's atmosphere to enable aerobic respiration?


A) Oxygenic photosynthesis by cyanobacteria significantly increased the amount of oxygen in the atmosphere.
B) Splitting of water during anaerobic respiration by cyanobacteria dramatically increased the amount of oxygen in the atmosphere.
C) Nitrogen fixation by cyanobacteria decreased the amount of nitrogen in the atmosphere, leading to the simultaneous increase of oxygen.
D) Anaerobic respiration by cyanobacteria increased the amount of oxygen in the atmosphere by reducing the amount of iron oxides that were able to react instantly with oxygen.

E) None of the above
F) A) and D)

Correct Answer

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You have found a new prokaryote. What line of evidence would support your hypothesis that the organism is a cyanobacterium?


A) It is able to form colonies and produce oxygen.
B) It is an endosymbiont.
C) It forms chains called mycelia.
D) You determine that it can cause Lyme disease.
E) It lacks cell walls.

F) B) and E)
G) A) and D)

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A water sample from a hot thermal vent contained a single-celled organism that had a cell wall but lacked a nucleus. What is its most likely classification?


A) Eukarya
B) Archaea
C) Animalia
D) Protista
E) Fungi

F) D) and E)
G) A) and E)

Correct Answer

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During cellular respiration, some bacteria use methane (CH4) as an electron donor and oxygen (O2) as an electron acceptor. What is the name for this group of bacteria?


A) methanogens
B) methanotrophs
C) organotrophs
D) nitrifiers

E) B) and C)
F) A) and D)

Correct Answer

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A prokaryote that obtains energy from light is a(n) ________.


A) chemotroph
B) phototroph
C) autotroph and phototroph
D) heterotroph and chemotroph
E) autotroph, phototroph, and chemotroph

F) A) and D)
G) A) and C)

Correct Answer

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Which of the following describe all existing bacteria?


A) pathogenic, omnipresent, morphologically diverse
B) extremophiles, tiny, abundant
C) small, harmful, fast-growing
D) tiny, ubiquitous, metabolically diverse
E) morphologically diverse, metabolically diverse, extremophiles

F) A) and C)
G) B) and D)

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