Question 1

What would be the 9-residue primer used to amplify:
5'-ATCGACGTTACGCTACATAGCATAAGGCTT-3'

Accepted Answer

A)   5'-TAGCTGCAA-3'
B)   5'-AAGCCTTAT-3'
C)   5'-UAGCUGCAA-3'
D)   5'-AAGCCUUAU-3'
E)   5'-TGCGATGTA-3'F) None of the above
G) C) and D)

Question 2

Which of the following is the appropriate source of the DNA polymerase included in the PCR reaction mixture?

Accepted Answer

A)   E. coli
B)   bacteriophage T4
C)   Thermus aquaticus
D)   Drosophila melanogaster
E)   HumanF) C) and E)
G) A) and B)

Question 3

Explain how the directed evolution of horseradish peroxidase was developed through high-throughput protein screening.​

Accepted Answer

Directed evolution involves mutation of ...

Question 4

To express a eukaryotic protein in E. coli, the eukaryotic cDNA must be cloned in a(n)  ____ that contains ____ and ____.

Accepted Answer

A)   expression vector; promoter; ribosomal-binding site
B)   hybridization complex; promoter; transition start site
C)   mRNA; ribosomal-binding site; promoter
D)   mRNA; introns; exons
E)   none of the aboveF) A) and E)
G) All of the above

Question 5

Which of the following describes the major utility of a eukaryotic cDNA library if one is trying to express a protein present in a eukaryotic cell?

Accepted Answer

A)   the library represents all genes that were actively expressed at a specific time
B)   the genes lack introns and thus can be expressed by bacterial expression systems
C)   cDNA libraries are readily available
D)   all of the above are correct
E)   both A and B are correctF) A) and B)
G) All of the above

Question 6

Each cycle of amplification in PCR involves all of the steps EXCEPT:

Accepted Answer

A)   the addition of fresh dNTPs to the reaction mixture.
B)   annealing of oligodeoxyribonucleotide primers to DNA.
C)   thermal denaturation of the target duplex DNA.
D)   reaction with DNA polymerase at approximately 70°C.
E)   none of the above.F) A) and B)
G) B) and E)

Question 7

All are steps in the sequence for construction of a chimeric plasmid EXCEPT:

Accepted Answer

A)   annealing the ends of the vector and foreign DNA.
B)   cutting the source of the foreign DNA with a restriction endonuclease.
C)   reannealing the ends of the vector back together.
D)   cutting the vector plasmid with the same restriction endonuclease.
E)   none of the above.F) A) and C)
G) None of the above

Question 8

To position a gene in an expression vector downstream from a promoter (i.e., perform directional cloning) , it is best to:

Accepted Answer

A)   use the largest plasmid available.
B)   treat the gene with DNase I.
C)   restrict both the gene and the plasmid with one restriction endonuclease and then screen all colonies for those that express the gene.
D)   restrict each end of the DNA fragment (gene)  with a different restriction endonuclease and do likewise for the plasmid.
E)   use a vector with two sites for the same restriction endonuclease.F) A) and B)
G) B) and C)

Question 9

PCR-based mutagenesis is used to create:

Accepted Answer

A)   mutant strains of an eukaryotic organism.
B)   generally modified plasmid vectors.
C)   mutant bacterial strains.
D)   mutant DNA libraries.
E)   specifically altered proteins.F) D) and E)
G) A) and D)

Question 10

In the Southern hybridization procedure, the gel after electrophoresis is treated with NaOH and then neutralized before blotting. What is the primary function of the alkaline treatment?

Accepted Answer

A)   It neutralizes any acid soluble impurities in the gel.
B)   It cleaves the DNA into smaller fragments to permit greater efficiency of transfer.
C)   It inactivates any restriction endonucleases that may be in the gel.
D)   It neutralizes any acidic phosphate groups that might prevent hybridization.
E)   It denatures the duplex DNA to single-stranded DNA (ssDN
A)  .F) B) and C)
G) B) and E)

Question 11

How does RNA interference function?

Accepted Answer

A)   siRNA binds to genes and prevents transcription
B)   a single strand of the siRNA binds to the gene transcript, preventing translation
C)   the double stranded siRNA binds to mRNA to prevent ribosomal association
D)   siRNA binds to RNA polymerase preventing mRNA production
E)   none of the aboveF) C) and D)
G) B) and D)

Question 12

All are characteristic of YACs (yeast artificial chromosomes)  EXCEPT:

Accepted Answer

A)   YACs can successfully propagate DNA molecules of 2 megabase pairs in length.
B)   YACs have been transferred into animals.
C)   YACs must include a centromere.
D)   YACs must include telomers.
E)   All are true.F) A) and E)
G) A) and D)

Question 13

All are features of a plasmid useful for cloning EXCEPT:

Accepted Answer

A)   expressed sequence tag.
B)   a cloning site.
C)   a replicator (origin of replication) .
D)   a selectable marker.
E)   all are features.F) All of the above
G) None of the above

Question 14

A method used to insert or transform cells with a plasmid is to:

Accepted Answer

A)   add the DNA to bacterial cells that have been lightly treated with lysozyme to produce "holes" in the cell wall.
B)   add the DNA to a heated suspension of cells at 42°C.
C)   treat the bacteria with Ca2+, add the DNA, and briefly heat to 42°C.
D)   incubate the DNA with the cells overnight at 4°C.
E)   mixing plasmids with an extract of broken cells.F) C) and D)
G) None of the above

Question 15

A genomic DNA library is:

Accepted Answer

A)   a collection of short fragments from nuclear DNA digestion.
B)   arrays of synthetic oligonucleotides used to select for a specific DNA.
C)   a set of cloned fragments that collectively represent the genes of a particular organism.
D)   a short segment of DNA whose sequence is complementary to a portion of the DNA of interest.
E)   a circular DNA molecule of 1 kb to 200 kb found in bacteria and yeast cells.F) D) and E)
G) A) and D)

Question 16

In vitro ____ makes it possible to alter the nucleotide sequence of a cloned gene systematically.

Accepted Answer

A)   protein synthesis
B)   mRNA synthesis
C)   hybridization
D)   mutagenesis
E)   all are trueF) C) and D)
G) B) and E)

Question 17

Define a probe and explain how probes are used in colony hybridization experiments.​

Accepted Answer

A probe is usually a single-stranded DNA...

Exam 12: Recombinant Dna, Cloning, Chimeric Genes, and Synthetic Biology

All are steps in the sequence for construction of a chimeric plasmid EXCEPT:

Correct Answer
verified

A genomic DNA library is:

Correct Answer
verified

To express a eukaryotic protein in E. coli, the eukaryotic cDNA must be cloned in a(n) that contains and .

Correct Answer
verified

To position a gene in an expression vector downstream from a promoter (i.e., perform directional cloning) , it is best to:

Correct Answer
verified

A method used to insert or transform cells with a plasmid is to:

Correct Answer
verified

All are features of a plasmid useful for cloning EXCEPT:

Correct Answer
verified

In vitro ____ makes it possible to alter the nucleotide sequence of a cloned gene systematically.

Correct Answer
verified

PCR-based mutagenesis is used to create:

Correct Answer
verified

How does RNA interference function?

Correct Answer
verified

Which of the following is the appropriate source of the DNA polymerase included in the PCR reaction mixture?

Correct Answer
verified

Which of the following describes the major utility of a eukaryotic cDNA library if one is trying to express a protein present in a eukaryotic cell?

Correct Answer
verified

What would be the 9-residue primer used to amplify: 5'-ATCGACGTTACGCTACATAGCATAAGGCTT-3'

Correct Answer
verified

All are characteristic of YACs (yeast artificial chromosomes) EXCEPT:

Correct Answer
verified

Explain how the directed evolution of horseradish peroxidase was developed through high-throughput protein screening.

Correct Answer
verified
Directed evolution involves mutation of ...
View Answer

In the Southern hybridization procedure, the gel after electrophoresis is treated with NaOH and then neutralized before blotting. What is the primary function of the alkaline treatment?

Correct Answer
verified

Define a probe and explain how probes are used in colony hybridization experiments.

Correct Answer
verified
A probe is usually a single-stranded DNA...
View Answer

Each cycle of amplification in PCR involves all of the steps EXCEPT:

Correct Answer
verified

Exam 12: Recombinant Dna, Cloning, Chimeric Genes, and Synthetic Biology

All are steps in the sequence for construction of a chimeric plasmid EXCEPT:

Correct AnswerverifiedShow Answer

A genomic DNA library is:

Correct AnswerverifiedShow Answer

To express a eukaryotic protein in E. coli, the eukaryotic cDNA must be cloned in a(n) ____ that contains ____ and ____.

Correct AnswerverifiedShow Answer

To position a gene in an expression vector downstream from a promoter (i.e., perform directional cloning) , it is best to:

Correct AnswerverifiedShow Answer

A method used to insert or transform cells with a plasmid is to:

Correct AnswerverifiedShow Answer

All are features of a plasmid useful for cloning EXCEPT:

Correct AnswerverifiedShow Answer

In vitro ____ makes it possible to alter the nucleotide sequence of a cloned gene systematically.

Correct AnswerverifiedShow Answer

PCR-based mutagenesis is used to create:

Correct AnswerverifiedShow Answer

How does RNA interference function?

Correct AnswerverifiedShow Answer

Which of the following is the appropriate source of the DNA polymerase included in the PCR reaction mixture?

Correct AnswerverifiedShow Answer

Which of the following describes the major utility of a eukaryotic cDNA library if one is trying to express a protein present in a eukaryotic cell?

Correct AnswerverifiedShow Answer

What would be the 9-residue primer used to amplify: 5'-ATCGACGTTACGCTACATAGCATAAGGCTT-3'

Correct AnswerverifiedShow Answer

All are characteristic of YACs (yeast artificial chromosomes) EXCEPT:

Correct AnswerverifiedShow Answer

Explain how the directed evolution of horseradish peroxidase was developed through high-throughput protein screening.​

Correct AnswerverifiedDirected evolution involves mutation of ...View AnswerShow Answer

In the Southern hybridization procedure, the gel after electrophoresis is treated with NaOH and then neutralized before blotting. What is the primary function of the alkaline treatment?

Correct AnswerverifiedShow Answer

Define a probe and explain how probes are used in colony hybridization experiments.​

Correct AnswerverifiedA probe is usually a single-stranded DNA...View AnswerShow Answer

Each cycle of amplification in PCR involves all of the steps EXCEPT:

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

To express a eukaryotic protein in E. coli, the eukaryotic cDNA must be cloned in a(n) that contains and .

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Explain how the directed evolution of horseradish peroxidase was developed through high-throughput protein screening.

Correct Answer
verified
Directed evolution involves mutation of ...
View Answer

Correct Answer
verified

Define a probe and explain how probes are used in colony hybridization experiments.

Correct Answer
verified
A probe is usually a single-stranded DNA...
View Answer

Correct Answer
verified